Automated GMP production and long-term experience in radiosynthesis of CB1 tracer [18F]FMPEP-d2

Salla Lahdenpohja, Thomas Keller, Sarita Forsback, Tapio Viljanen, Esa Kokkomäki, Riikka V. Kivelä, Jörgen Bergman, Olof Solin, Anna K. Kirjavainen

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

Here, we describe the development of an in-house-built device for the fully automated multistep synthesis of the cannabinoid CB1 receptor imaging tracer (3R,5R)-5-(3-([18F]fluoromethoxy-d2)phenyl)-3-(((R)-1-phenylethyl)amino)-1-(4-(trifluoromethyl)phenyl)pyrrolidin-2-one ([18F]FMPEP-d2), following good manufacturing practices. The device is interfaced to a HPLC and a sterile filtration unit in a clean room hot cell. The synthesis involves the nucleophilic 18F-fluorination of an alkylating agent and its GC purification, the subsequent 18F-fluoroalkylation of a precursor molecule, the semipreparative HPLC purification of the 18F-fluoroalkylated product, and its formulation for injection. We have optimized the duration and temperature of the 18F-fluoroalkylation reaction and addressed the radiochemical stability of the formulated product. During the past 5 years (2013?2018), we have performed a total of 149 syntheses for clinical use with a 90% success rate. The activity yield of the formulated product has been 1.0 ± 0.4 GBq starting from 11 ± 2 GBq and the molar activity 600 ± 300 GBq/?mol at the end of synthesis.
Original languageEnglish
Pages (from-to)408-418
Number of pages11
JournalJournal of Labelled Compounds and Radiopharmaceuticals
Volume63
Issue number9
DOIs
Publication statusPublished - 2020
MoE publication typeA1 Journal article-refereed

Keywords

  • fluoroalkylation
  • GMP
  • nucleophilic 18F-fluorination
  • positron emission tomography
  • radiochemistry
  • radiopharmaceutical

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