Aqueous Two-Phase Emulsion Bioresin for Facile One-Step 3D Microgel-Based Bioprinting

Qingbo Wang; Özge Karadas; Oskar Backman; Luyao Wang; Tuomas Näreoja; Jessica M. Rosenholm; Chunlin Xu; Xiaoju Wang

doi:10.1002/adhm.202203243

Aqueous Two-Phase Emulsion Bioresin for Facile One-Step 3D Microgel-Based Bioprinting

Qingbo Wang
, Özge Karadas
, Oskar Backman
, Luyao Wang
, Tuomas Näreoja
, Jessica M. Rosenholm
, Chunlin Xu
, Xiaoju Wang^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

34 Citations (Scopus)

201 Downloads (Pure)

Abstract

Microgel assembly as void-forming bioinks in 3D bioprinting has evidenced recent success with a highlighted scaffolding performance of these bottom-up biomaterial systems in supporting the viability and function of the laden cells. Here, a ternary-component aqueous emulsion is established as a one-step strategy to integrate the methacrylated gelatin (GelMA) microgel fabrication and assembly through vat photopolymerization in situ using digital light processing (DLP)-based bioprinting. The as-proposed aqueous emulsion is featured with the partitioning of a secondary photo-crosslinkable polysaccharide, methacrylated galactoglucomannan (GGMMA) derived from plant source in both the dispersed phase of GelMA droplets and the continuous phase of dextran (Dex). As an emulgator, GGMMA renders enhanced stability of the aqueous emulsion bioresins. Strategically, the photo-crosslinkable GGMMA adheres the GelMA microgels that are conveniently converted from emulsion droplets to form hydrogel construct in layer-by-layer curing to accommodate the laden cells directly mixed in the aqueous emulsion. The spatially interconnected void space left by the removal of Dex benefits the cell growth under the guidance of the microgel surface and supports cell colonization within the macroscopic porous hydrogel. This work amends a low-concentration and cost-effective bioresin that is highly applicable for facilely fabricating microgel assembly as a porous hydrogel construct in DLP-based bioprinting.

Original language	English
Article number	2203243
Number of pages	15
Journal	Advanced Healthcare Materials
Volume	12
Issue number	19
DOIs	https://doi.org/10.1002/adhm.202203243
Publication status	Published - 27 Jul 2023
MoE publication type	A1 Journal article-refereed

Funding

Q.W. would like to acknowledge the financial support from the China Scholarship Council (Student ID 201907960002) and the Finnish Ministry of Education and Culture funded international pilot project “Finland‐China Food and Health Network” to his doctoral study at Åbo Akademi University (ÅAU), Finland. X.W. would like to thank the Academy of Finland (333158) as well as the Jane and Aatos Erkko Foundation for their funds for her research at ÅAU. T.N. and Ö.K. would like to thank the Olins Foundation within Swedish Cultural Foundation in Finland and the Finnish Society for Sciences for supporting their research. J.M.R.and C.X. acknowledge the REACT EU funding received from the European Regional Development Fund (ERDF) for the project “AMBioPharma” (Centre for Additive Manufacturing for Life Science and Pharmaceutical Industry, project code A77805).

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being
SDG 9 Industry, Innovation, and Infrastructure
SDG 12 Responsible Consumption and Production

Keywords

3D bioprinting
aqueous emulsion
microgel assembly
microporous hydrogels
vat photopolymerization

Access to Document

10.1002/adhm.202203243Licence: CC BY

Adv Healthcare Materials - 2023 - Wang - Aqueous Two‐Phase Emulsion Bioresin for Facile One‐Step 3D Microgel‐BasedFinal published version, 4.5 MBLicence: CC BY

https://urn.fi/URN:NBN:fi-fe2024120198215

Cite this

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title = "Aqueous Two-Phase Emulsion Bioresin for Facile One-Step 3D Microgel-Based Bioprinting",

abstract = "Microgel assembly as void-forming bioinks in 3D bioprinting has evidenced recent success with a highlighted scaffolding performance of these bottom-up biomaterial systems in supporting the viability and function of the laden cells. Here, a ternary-component aqueous emulsion is established as a one-step strategy to integrate the methacrylated gelatin (GelMA) microgel fabrication and assembly through vat photopolymerization in situ using digital light processing (DLP)-based bioprinting. The as-proposed aqueous emulsion is featured with the partitioning of a secondary photo-crosslinkable polysaccharide, methacrylated galactoglucomannan (GGMMA) derived from plant source in both the dispersed phase of GelMA droplets and the continuous phase of dextran (Dex). As an emulgator, GGMMA renders enhanced stability of the aqueous emulsion bioresins. Strategically, the photo-crosslinkable GGMMA adheres the GelMA microgels that are conveniently converted from emulsion droplets to form hydrogel construct in layer-by-layer curing to accommodate the laden cells directly mixed in the aqueous emulsion. The spatially interconnected void space left by the removal of Dex benefits the cell growth under the guidance of the microgel surface and supports cell colonization within the macroscopic porous hydrogel. This work amends a low-concentration and cost-effective bioresin that is highly applicable for facilely fabricating microgel assembly as a porous hydrogel construct in DLP-based bioprinting.",

keywords = "3D bioprinting, aqueous emulsion, microgel assembly, microporous hydrogels, vat photopolymerization",

author = "Qingbo Wang and {\"O}zge Karadas and Oskar Backman and Luyao Wang and Tuomas N{\"a}reoja and Rosenholm, \{Jessica M.\} and Chunlin Xu and Xiaoju Wang",

note = "Funding Information: Q.W. would like to acknowledge the financial support from the China Scholarship Council (Student ID 201907960002) and the Finnish Ministry of Education and Culture funded international pilot project {\textquotedblleft}Finland‐China Food and Health Network{\textquotedblright} to his doctoral study at {\AA}bo Akademi University ({\AA}AU), Finland. X.W. would like to thank the Academy of Finland (333158) as well as the Jane and Aatos Erkko Foundation for their funds for her research at {\AA}AU. T.N. and {\"O}.K. would like to thank the Olins Foundation within Swedish Cultural Foundation in Finland and the Finnish Society for Sciences for supporting their research. J.M.R.and C.X. acknowledge the REACT EU funding received from the European Regional Development Fund (ERDF) for the project {\textquotedblleft}AMBioPharma{\textquotedblright} (Centre for Additive Manufacturing for Life Science and Pharmaceutical Industry, project code A77805). Publisher Copyright: {\textcopyright} 2023 Wiley-VCH GmbH.",

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AU - Wang, Qingbo

AU - Karadas, Özge

AU - Backman, Oskar

AU - Wang, Luyao

AU - Näreoja, Tuomas

AU - Rosenholm, Jessica M.

AU - Xu, Chunlin

AU - Wang, Xiaoju

N1 - Funding Information: Q.W. would like to acknowledge the financial support from the China Scholarship Council (Student ID 201907960002) and the Finnish Ministry of Education and Culture funded international pilot project “Finland‐China Food and Health Network” to his doctoral study at Åbo Akademi University (ÅAU), Finland. X.W. would like to thank the Academy of Finland (333158) as well as the Jane and Aatos Erkko Foundation for their funds for her research at ÅAU. T.N. and Ö.K. would like to thank the Olins Foundation within Swedish Cultural Foundation in Finland and the Finnish Society for Sciences for supporting their research. J.M.R.and C.X. acknowledge the REACT EU funding received from the European Regional Development Fund (ERDF) for the project “AMBioPharma” (Centre for Additive Manufacturing for Life Science and Pharmaceutical Industry, project code A77805). Publisher Copyright: © 2023 Wiley-VCH GmbH.

PY - 2023/7/27

Y1 - 2023/7/27

N2 - Microgel assembly as void-forming bioinks in 3D bioprinting has evidenced recent success with a highlighted scaffolding performance of these bottom-up biomaterial systems in supporting the viability and function of the laden cells. Here, a ternary-component aqueous emulsion is established as a one-step strategy to integrate the methacrylated gelatin (GelMA) microgel fabrication and assembly through vat photopolymerization in situ using digital light processing (DLP)-based bioprinting. The as-proposed aqueous emulsion is featured with the partitioning of a secondary photo-crosslinkable polysaccharide, methacrylated galactoglucomannan (GGMMA) derived from plant source in both the dispersed phase of GelMA droplets and the continuous phase of dextran (Dex). As an emulgator, GGMMA renders enhanced stability of the aqueous emulsion bioresins. Strategically, the photo-crosslinkable GGMMA adheres the GelMA microgels that are conveniently converted from emulsion droplets to form hydrogel construct in layer-by-layer curing to accommodate the laden cells directly mixed in the aqueous emulsion. The spatially interconnected void space left by the removal of Dex benefits the cell growth under the guidance of the microgel surface and supports cell colonization within the macroscopic porous hydrogel. This work amends a low-concentration and cost-effective bioresin that is highly applicable for facilely fabricating microgel assembly as a porous hydrogel construct in DLP-based bioprinting.

AB - Microgel assembly as void-forming bioinks in 3D bioprinting has evidenced recent success with a highlighted scaffolding performance of these bottom-up biomaterial systems in supporting the viability and function of the laden cells. Here, a ternary-component aqueous emulsion is established as a one-step strategy to integrate the methacrylated gelatin (GelMA) microgel fabrication and assembly through vat photopolymerization in situ using digital light processing (DLP)-based bioprinting. The as-proposed aqueous emulsion is featured with the partitioning of a secondary photo-crosslinkable polysaccharide, methacrylated galactoglucomannan (GGMMA) derived from plant source in both the dispersed phase of GelMA droplets and the continuous phase of dextran (Dex). As an emulgator, GGMMA renders enhanced stability of the aqueous emulsion bioresins. Strategically, the photo-crosslinkable GGMMA adheres the GelMA microgels that are conveniently converted from emulsion droplets to form hydrogel construct in layer-by-layer curing to accommodate the laden cells directly mixed in the aqueous emulsion. The spatially interconnected void space left by the removal of Dex benefits the cell growth under the guidance of the microgel surface and supports cell colonization within the macroscopic porous hydrogel. This work amends a low-concentration and cost-effective bioresin that is highly applicable for facilely fabricating microgel assembly as a porous hydrogel construct in DLP-based bioprinting.

KW - 3D bioprinting

KW - aqueous emulsion

KW - microgel assembly

KW - microporous hydrogels

KW - vat photopolymerization

UR - https://www.scopus.com/pages/publications/85150922744

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DO - 10.1002/adhm.202203243

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SN - 2192-2640

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JO - Advanced Healthcare Materials

JF - Advanced Healthcare Materials

IS - 19

M1 - 2203243

ER -

Aqueous Two-Phase Emulsion Bioresin for Facile One-Step 3D Microgel-Based Bioprinting

Abstract

Funding

UN SDGs

Keywords

Access to Document

Other files and links

Fingerprint

Åbo Akademi Functional Printing Center

AMBioPharma: Centre for Additive Manufacturing for Life Science and Pharmaceutical Industry

Cite this

Aqueous Two-Phase Emulsion Bioresin for Facile One-Step 3D Microgel-Based Bioprinting

Abstract

Funding

UN SDGs

Keywords

Access to Document

Other files and links

Fingerprint

Equipment

Åbo Akademi Functional Printing Center

Projects

AMBioPharma: Centre for Additive Manufacturing for Life Science and Pharmaceutical Industry

Cite this