Application of Paper-Supported Printed Gold Electrodes for Impedimetric Immunosensor Development

Petri Ihalainen*, Himadri Majumdar, Tapani Viitala, Björn Törngren, Tuomas Närjeoja, Anni Määttänen, Jawad Sarfraz, Harri Härmä, Marjo Yliperttula, Ronald Österbacka, Jouko Peltonen

*Corresponding author for this work

    Research output: Contribution to journalArticleScientificpeer-review

    20 Citations (Scopus)

    Abstract

    In this article, we report on the formation and mode-of-operation of an affinity biosensor, where alternate layers of biotin/streptavidin/biotinylated-CRP-antigen/anti-CRP antibody are grown on printed gold electrodes on disposable paper-substrates. We have successfully demonstrated and detected the formation of consecutive layers of supra-molecular protein assembly using an electrical (impedimetric) technique. The formation process is also supplemented and verified using conventional surface plasmon resonance (SPR) measurements and surface sensitive characterization techniques, such as X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The article provides a possible biosensor development scheme, where-(1) fabrication of paper substrate (2) synthesis of gold nanoparticle inks (3) inkjet printing of gold electrodes on paper (4) formation of the biorecognition layers on the gold electrodes and (5) electrical (impedimetric) analysis of growth-all are coupled together to form a test-structure for a recyclable and inexpensive point-of-care diagnostic platform.

    Original languageEnglish
    Pages (from-to)1-17
    Number of pages17
    JournalBiosensors
    Volume3
    Issue number1
    DOIs
    Publication statusPublished - Mar 2013
    MoE publication typeA1 Journal article-refereed

    Keywords

    • Immunoassays
    • Impedance spectroscopy
    • Inkjet printing
    • Nanoparticles
    • Paper electronics

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