Anaphase-Promoting Complex/Cyclosome Participates in the Acute Response to Protein-Damaging Stress

JK Ahlskog, Johanna Björk, Alexandra Elsing, Camilla Aspelin, Marko Kallio, Pia Roos-Mattjus, Lea Sistonen

    Research output: Contribution to journalArticleScientificpeer-review

    22 Citations (Scopus)

    Abstract

    The ubiquitin E3 ligase anaphase-promoting complex/cyclosome (APC/C) drives degradation of cell cycle regulators in cycling cells by associating with the coactivators Cdc20 and Cdh1. Although a plethora of APC/C substrates have been identified, only a few transcriptional regulators are described as direct targets of APC/C-dependent ubiquitination. Here we show that APC/C, through substrate recognition by both Cdc20 and Cdh1, mediates ubiquitination and degradation of heat shock factor 2 (HSF2), a transcription factor that binds to the Hsp70 promoter. The interaction between HSF2 and the APC/C subunit Cdc27 and coactivator Cdc20 is enhanced by moderate heat stress, and the degradation of HSF2 is induced during the acute phase of the heat shock response, leading to clearance of HSF2 from the Hsp70 promoter. Remarkably, Cdc20 and the proteasome 20S core alpha 2 subunit are recruited to the Hsp70 promoter in a heat shock-inducible manner. Moreover, the heat shock-induced expression of Hsp70 is increased when Cdc20 is silenced by a specific small interfering RNA (siRNA). Our results provide the first evidence for participation of APC/C in the acute response to protein-damaging stress.
    Original languageUndefined/Unknown
    Pages (from-to)5608–5620
    Number of pages13
    JournalMolecular and Cellular Biology
    Volume30
    Issue number24
    DOIs
    Publication statusPublished - 2010
    MoE publication typeA1 Journal article-refereed

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