Analysis of tyrosine kinase mRNAs including four FGF receptor mRNAs expressed in MCF-7 breast-cancer cells

L Lehtola, Juha Partanen, L Sistonen, J Korhonen, A Wärri, P Härkönen, R Clarke, K Alitalo

Research output: Contribution to journalArticleScientificpeer-review

45 Citations (Scopus)

Abstract

The MCF-7 cell line is a hormone-responsive human breast-cancer cell line, which has been extensively used in studies of estrogen regulation of cell growth. These studies have indicated that the growth stimulation of the MCF-7 cells by estrogens may be effected by an autocrine mechanism involving several growth factors, such as EGF, TGF alpha and IGF-I and their receptors. We have amplified and cloned tyrosine-kinase-related sequences from the MCF-7 cell mRNA using the polymerase chain reaction and characterized the partial cDNAs obtained by nucleic acid sequencing. Nine tyrosine kinase cDNAs and one serine/threonine kinase cDNA were identified among the amplified sequences. Four different tyrosine kinase genes encoding receptors for fibroblast growth factors (FGFs) were found to be expressed by the MCF-7 cells. In addition, differences were observed in the expression of these members of FGF receptor family in different breast-cancer cells. A putative tyrosine-kinase receptor and a novel serine/threonine kinase were preferentially expressed in estrogen-responsive tumor cell lines. However, no estrogen-dependent regulation of any of the novel tyrosine-kinase receptor mRNAs was found in any of the cell lines including the MCF-7 or ZR-75-I cells, where the expression of the neu proto-oncogene mRNA was decreased during estrogen treatment. The expression of several FGF receptors by breast-cancer cells suggests that FGFs may be involved in their growth regulation and tumorigenesis.

Original languageEnglish
Pages (from-to)598-603
Number of pages6
JournalInternational Journal of Cancer
Volume50
Issue number4
DOIs
Publication statusPublished - 20 Feb 1992
MoE publication typeA1 Journal article-refereed

Keywords

  • Amino Acid Sequence
  • Base Sequence
  • Breast Neoplasms/genetics
  • Cloning, Molecular
  • Gene Expression
  • Gene Expression Regulation, Neoplastic/drug effects
  • Humans
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides/chemistry
  • Polymerase Chain Reaction
  • Protein Kinase C/genetics
  • Protein Kinases/genetics
  • Protein Serine-Threonine Kinases
  • Protein-Tyrosine Kinases/genetics
  • Proto-Oncogene Mas
  • RNA, Messenger/genetics
  • RNA, Neoplasm/genetics
  • Receptors, Cell Surface/genetics
  • Receptors, Fibroblast Growth Factor
  • Sequence Alignment
  • Steroids/pharmacology
  • Tumor Cells, Cultured

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