Analysis of Label-Free Single-Molecule Biosensors based on Gate-Biofunctionalized Organic Transistors

F Torricelli, Eleonora Macchia, K Manoli, Di Franco C, ZM Kovacs-Vajna, G Palazzo, G Scamarcio, Luisa Torsi

Research output: Chapter in Book/Conference proceedingConference contributionScientificpeer-review


A label-free single-molecule detection platform based on biofunctionalized organic transistors has been recently proposed. In contrast to state-of-art approaches based on nano-transducers, the aforementioned single-molecule transistor (SiMoT) sensing technology is based on millimeter-sized transistors where the gate is bio-functionalized with about a trillion of antibodies. In this work we provide a detailed investigation of SiMoT biosensors by studying and quantifying the device parameters affected by the biorecognition event. The study combines both measurements and theoretical analyses, showing that a single protein detection taking place at nanometer scale affects a millimeter scale area of the biofunctionalized gate electrode. The dimension of the bioprobe domains affected by the binding is calculated as a function of the nominal protein concentration in the analyte solution. These results provide important insight into the SiMoT biosensor technology, which can guide the development of multi-modal SiMoT detection and integrated SiMoT biosensors arrays.
Original languageUndefined/Unknown
Title of host publication2019 IEEE 8th International Workshop on Advances in Sensors and Interfaces (IWASI)
Number of pages5
ISBN (Electronic)978-1-7281-0557-4
ISBN (Print)978-1-7281-0558-1
Publication statusPublished - 2019
MoE publication typeA4 Article in a conference publication
EventInternational Workshop on Advances in Sensors and Interfaces - 8th International Workshop on Advances in Sensors and Interfaces
Duration: 13 Jun 201914 Jun 2019


ConferenceInternational Workshop on Advances in Sensors and Interfaces


  • biofunctionalization
  • biosensor
  • organic transistor
  • single molecule

Cite this