Analysis of Label-Free Single-Molecule Biosensors based on Gate-Biofunctionalized Organic Transistors

F Torricelli, Eleonora Macchia, K Manoli, Di Franco C, ZM Kovacs-Vajna, G Palazzo, G Scamarcio, Luisa Torsi

    Research output: Chapter in Book/Conference proceedingConference contributionScientificpeer-review

    Abstract

    A label-free single-molecule detection platform based on biofunctionalized organic transistors has been recently proposed. In contrast to state-of-art approaches based on nano-transducers, the aforementioned single-molecule transistor (SiMoT) sensing technology is based on millimeter-sized transistors where the gate is bio-functionalized with about a trillion of antibodies. In this work we provide a detailed investigation of SiMoT biosensors by studying and quantifying the device parameters affected by the biorecognition event. The study combines both measurements and theoretical analyses, showing that a single protein detection taking place at nanometer scale affects a millimeter scale area of the biofunctionalized gate electrode. The dimension of the bioprobe domains affected by the binding is calculated as a function of the nominal protein concentration in the analyte solution. These results provide important insight into the SiMoT biosensor technology, which can guide the development of multi-modal SiMoT detection and integrated SiMoT biosensors arrays.
    Original languageUndefined/Unknown
    Title of host publication2019 IEEE 8th International Workshop on Advances in Sensors and Interfaces (IWASI)
    PublisherIEEE
    Pages70–74
    Number of pages5
    ISBN (Electronic)978-1-7281-0557-4
    ISBN (Print)978-1-7281-0558-1
    DOIs
    Publication statusPublished - 2019
    MoE publication typeA4 Article in a conference publication
    EventInternational Workshop on Advances in Sensors and Interfaces - 8th International Workshop on Advances in Sensors and Interfaces
    Duration: 13 Jun 201914 Jun 2019

    Conference

    ConferenceInternational Workshop on Advances in Sensors and Interfaces
    Period13/06/1914/06/19

    Keywords

    • biofunctionalization
    • biosensor
    • organic transistor
    • single molecule

    Cite this