Analysis of extractives from Pinus halepensis and Eucalyptus camaldulensis as predominant trees in Algeria

Nacera Benouadah, Andrey Pranovich, Djamel Aliouche, Jarl Hemming, Annika Smeds, Stefan Willför

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The lipophilic and hydrophilic extractives in the sapwood (sW) and heartwood (hW) of stems from Pinus halepensis Mill and Eucalyptus camaldulensis Dehnh trees grown in the north of Algeria were analyzed. The extraction of dried samples was carried out in an accelerated solvent extractor (ASE). The lipophilic substances were first extracted with n-hexane and then the hydrophilic ones with acetone/water. The extractives were analyzed by gas chromatography-flame ionization detection (GC-FID), GC-mass spectroscopy (MS) and high-performance sizeexclusion chromatography (HPSEC). The largest amount of lipophilic extractives (≈13.4 mg g−1) was observed in the hW of P. halepensis, while the hW of E. camaldulensis contained the largest amount of hydrophilic extractives (≈116.3 mg g−1). Lipophilic extractives are mainly composed of oleoresins (resin acids, terpenes), fats (fatty acids, glycerides, steryl esters, sterols) and waxes (fatty alcohols). Hydrophilic extractives are composed of polyphenols (stilbenes, flavanols), sugars (monosaccharides) and sugar alcohols (cyclic polyols). The main identified lipophilic extractives are resin acids in pine and glycerides in eucalypt. The main identified hydrophilic extractives are cyclic polyols in pine and flavanols and monosaccharides in eucalypt. The total content of extractives is higher in hW than in sW.

Original languageUndefined/Unknown
Pages (from-to)97–104
Issue number2
Publication statusPublished - 2018
MoE publication typeA1 Journal article-refereed


  • heartwood
  • Pinus halepensis
  • lipophilic
  • extractives
  • sapwood
  • Eucalyptus camaldulensis
  • hydrophilic

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