Advances in UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) covalent inhibition

Maycon V. de Oliveira, Renan M. Furtado, Kauê S. da Costa, Serhii Vakal, Anderson H. Lima

    Research output: Contribution to journalReview Article or Literature Reviewpeer-review

    5 Citations (Scopus)
    19 Downloads (Pure)

    Abstract

    Peptidoglycan is a cross-linked polymer responsible for maintaining the bacterial cell wall integrity and morphology in Gram-negative and Gram-positive bacteria. The peptidoglycan pathway consists of the enzymatic reactions held in three steps: cytoplasmic, membrane-associated, and periplasmic. The Mur enzymes (MurA-MurF) are involved in a cytoplasmic stage. The UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) enzyme is responsible for transferring the enolpyruvate group from phosphoenolpyruvate (PEP) to UDP-N-acetylglucosamine (UNAG) to form UDP-N-acetylglucosamine enolpyruvate (EP-UNAG). Fosfomycin is a natural product analogous to PEP that acts on the MurA target enzyme via binding covalently to the key cysteine residue in the active site. Similar to fosfomycin, other MurA covalent inhibitors have been described with a warhead in their structure that forms a covalent bond with the molecular target. In MurA, the nucleophilic thiolate of Cys115 is pointed as the main group involved in the warhead binding. Thus, in this minireview, we briefly describe the main recent advances in the design of MurA covalent inhibitors.

    Original languageEnglish
    JournalFrontiers in Molecular Biosciences
    Volume9
    DOIs
    Publication statusPublished - 20 Jul 2022
    MoE publication typeA2 Review article in a scientific journal

    Keywords

    • Covalent inhibitors
    • Bacterial resistance
    • Fosfomycin
    • Peptidoglycan
    • MurA enzyme

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