Accumulation of free and covalently bound microcystins in tissues of Lymnaea stagnalis (Gastropoda) following toxic cyanobacteria or dissolved microcystin-LR exposure

Emilie Lance; Milla Riina Neffling; Claudia Gérard; Jussi Meriluoto; Myriam Bormans

doi:10.1016/j.envpol.2009.10.025

Accumulation of free and covalently bound microcystins in tissues of Lymnaea stagnalis (Gastropoda) following toxic cyanobacteria or dissolved microcystin-LR exposure

Emilie Lance^*
, Milla Riina Neffling
, Claudia Gérard
, Jussi Meriluoto
, Myriam Bormans

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

63 Citations (Scopus)

Abstract

Accumulation of free microcystins (MCs) in freshwater gastropods has been demonstrated but accumulation of MCs covalently bound to tissues has never been considered so far. Here, we follow the accumulation of total (free and bound) MCs in Lymnaea stagnalis exposed to i) dissolved MC-LR (33 and 100 μg L^-1) and ii) Planktothrix agardhii suspensions producing 5 and 33 μg MC-LR equivalents L^-1 over a 5-week period, and after a 3-week depuration period. Snails exposed to dissolved MC-LR accumulated up to 0.26 μg total MCs g^-1 dry weight (DW), with no detection of bound MCs. Snails exposed to MCs producing P. agardhii accumulated up to 69.9 μg total MCs g^-1 DW, of which from 17.7 to 66.7% were bound. After depuration, up to 15.3 μg g^-1 DW of bound MCs were detected in snails previously exposed to toxic cyanobacteria, representing a potential source of MCs transfer through the food web.

Original language	English
Pages (from-to)	674-680
Number of pages	7
Journal	Environmental Pollution
Volume	158
Issue number	3
DOIs	https://doi.org/10.1016/j.envpol.2009.10.025
Publication status	Published - Mar 2010
MoE publication type	A1 Journal article-refereed

Funding

Authors thank the Institut National de Recherche en Agronomie (Rennes, France) for providing individuals L. stagnalis and the Museum National d'Histoire Naturelle (Paris, France) for providing the P. agardhii PMC 75-02 strain. Authors are grateful to the CIMO (Center for International Mobility) and to the French and Finnish Ministries of Foreign Affairs, which partly supported this work through a 3-month fellowship to E. Lance for a stay in Finland, in the laboratory of Dr. J. Meriluoto. M-R Neffling acknowledges the National graduate school in Informational and Structural Biology (ISB) and Academy of Finland, decision number 108947 for funding.

Keywords

Accumulation
Covalently bound microcystins
Cyanobacteria
Detoxification
Gastropods

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10.1016/j.envpol.2009.10.025

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@article{72518c214ffa4d0596d4fd972cea5744,

title = "Accumulation of free and covalently bound microcystins in tissues of Lymnaea stagnalis (Gastropoda) following toxic cyanobacteria or dissolved microcystin-LR exposure",

abstract = "Accumulation of free microcystins (MCs) in freshwater gastropods has been demonstrated but accumulation of MCs covalently bound to tissues has never been considered so far. Here, we follow the accumulation of total (free and bound) MCs in Lymnaea stagnalis exposed to i) dissolved MC-LR (33 and 100 μg L-1) and ii) Planktothrix agardhii suspensions producing 5 and 33 μg MC-LR equivalents L-1 over a 5-week period, and after a 3-week depuration period. Snails exposed to dissolved MC-LR accumulated up to 0.26 μg total MCs g-1 dry weight (DW), with no detection of bound MCs. Snails exposed to MCs producing P. agardhii accumulated up to 69.9 μg total MCs g-1 DW, of which from 17.7 to 66.7\% were bound. After depuration, up to 15.3 μg g-1 DW of bound MCs were detected in snails previously exposed to toxic cyanobacteria, representing a potential source of MCs transfer through the food web.",

keywords = "Accumulation, Covalently bound microcystins, Cyanobacteria, Detoxification, Gastropods",

author = "Emilie Lance and Neffling, \{Milla Riina\} and Claudia G{\'e}rard and Jussi Meriluoto and Myriam Bormans",

note = "Funding Information: Authors thank the Institut National de Recherche en Agronomie (Rennes, France) for providing individuals L. stagnalis and the Museum National d'Histoire Naturelle (Paris, France) for providing the P. agardhii PMC 75-02 strain. Authors are grateful to the CIMO (Center for International Mobility) and to the French and Finnish Ministries of Foreign Affairs, which partly supported this work through a 3-month fellowship to E. Lance for a stay in Finland, in the laboratory of Dr. J. Meriluoto. M-R Neffling acknowledges the National graduate school in Informational and Structural Biology (ISB) and Academy of Finland, decision number 108947 for funding. Copyright: Copyright 2010 Elsevier B.V., All rights reserved.",

year = "2010",

month = mar,

doi = "10.1016/j.envpol.2009.10.025",

language = "English",

volume = "158",

pages = "674--680",

journal = "Environmental Pollution",

issn = "0269-7491",

publisher = "Elsevier",

number = "3",

}

Accumulation of free and covalently bound microcystins in tissues of Lymnaea stagnalis (Gastropoda) following toxic cyanobacteria or dissolved microcystin-LR exposure. / Lance, Emilie; Neffling, Milla Riina; Gérard, Claudia et al.
In: Environmental Pollution, Vol. 158, No. 3, 03.2010, p. 674-680.

Research output: Contribution to journal › Article › Scientific › peer-review

TY - JOUR

T1 - Accumulation of free and covalently bound microcystins in tissues of Lymnaea stagnalis (Gastropoda) following toxic cyanobacteria or dissolved microcystin-LR exposure

AU - Lance, Emilie

AU - Neffling, Milla Riina

AU - Gérard, Claudia

AU - Meriluoto, Jussi

AU - Bormans, Myriam

N1 - Funding Information: Authors thank the Institut National de Recherche en Agronomie (Rennes, France) for providing individuals L. stagnalis and the Museum National d'Histoire Naturelle (Paris, France) for providing the P. agardhii PMC 75-02 strain. Authors are grateful to the CIMO (Center for International Mobility) and to the French and Finnish Ministries of Foreign Affairs, which partly supported this work through a 3-month fellowship to E. Lance for a stay in Finland, in the laboratory of Dr. J. Meriluoto. M-R Neffling acknowledges the National graduate school in Informational and Structural Biology (ISB) and Academy of Finland, decision number 108947 for funding. Copyright: Copyright 2010 Elsevier B.V., All rights reserved.

PY - 2010/3

Y1 - 2010/3

N2 - Accumulation of free microcystins (MCs) in freshwater gastropods has been demonstrated but accumulation of MCs covalently bound to tissues has never been considered so far. Here, we follow the accumulation of total (free and bound) MCs in Lymnaea stagnalis exposed to i) dissolved MC-LR (33 and 100 μg L-1) and ii) Planktothrix agardhii suspensions producing 5 and 33 μg MC-LR equivalents L-1 over a 5-week period, and after a 3-week depuration period. Snails exposed to dissolved MC-LR accumulated up to 0.26 μg total MCs g-1 dry weight (DW), with no detection of bound MCs. Snails exposed to MCs producing P. agardhii accumulated up to 69.9 μg total MCs g-1 DW, of which from 17.7 to 66.7% were bound. After depuration, up to 15.3 μg g-1 DW of bound MCs were detected in snails previously exposed to toxic cyanobacteria, representing a potential source of MCs transfer through the food web.

AB - Accumulation of free microcystins (MCs) in freshwater gastropods has been demonstrated but accumulation of MCs covalently bound to tissues has never been considered so far. Here, we follow the accumulation of total (free and bound) MCs in Lymnaea stagnalis exposed to i) dissolved MC-LR (33 and 100 μg L-1) and ii) Planktothrix agardhii suspensions producing 5 and 33 μg MC-LR equivalents L-1 over a 5-week period, and after a 3-week depuration period. Snails exposed to dissolved MC-LR accumulated up to 0.26 μg total MCs g-1 dry weight (DW), with no detection of bound MCs. Snails exposed to MCs producing P. agardhii accumulated up to 69.9 μg total MCs g-1 DW, of which from 17.7 to 66.7% were bound. After depuration, up to 15.3 μg g-1 DW of bound MCs were detected in snails previously exposed to toxic cyanobacteria, representing a potential source of MCs transfer through the food web.

KW - Accumulation

KW - Covalently bound microcystins

KW - Cyanobacteria

KW - Detoxification

KW - Gastropods

UR - https://www.scopus.com/pages/publications/74149087519

U2 - 10.1016/j.envpol.2009.10.025

DO - 10.1016/j.envpol.2009.10.025

M3 - Article

C2 - 19906474

AN - SCOPUS:74149087519

SN - 0269-7491

VL - 158

SP - 674

EP - 680

JO - Environmental Pollution

JF - Environmental Pollution

IS - 3

ER -

Accumulation of free and covalently bound microcystins in tissues of Lymnaea stagnalis (Gastropoda) following toxic cyanobacteria or dissolved microcystin-LR exposure

Abstract

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Keywords

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