TY - JOUR
T1 - Accumulation of free and covalently bound microcystins in tissues of Lymnaea stagnalis (Gastropoda) following toxic cyanobacteria or dissolved microcystin-LR exposure
AU - Lance, Emilie
AU - Neffling, Milla Riina
AU - Gérard, Claudia
AU - Meriluoto, Jussi
AU - Bormans, Myriam
N1 - Funding Information:
Authors thank the Institut National de Recherche en Agronomie (Rennes, France) for providing individuals L. stagnalis and the Museum National d'Histoire Naturelle (Paris, France) for providing the P. agardhii PMC 75-02 strain. Authors are grateful to the CIMO (Center for International Mobility) and to the French and Finnish Ministries of Foreign Affairs, which partly supported this work through a 3-month fellowship to E. Lance for a stay in Finland, in the laboratory of Dr. J. Meriluoto. M-R Neffling acknowledges the National graduate school in Informational and Structural Biology (ISB) and Academy of Finland, decision number 108947 for funding.
Copyright:
Copyright 2010 Elsevier B.V., All rights reserved.
PY - 2010/3
Y1 - 2010/3
N2 - Accumulation of free microcystins (MCs) in freshwater gastropods has been demonstrated but accumulation of MCs covalently bound to tissues has never been considered so far. Here, we follow the accumulation of total (free and bound) MCs in Lymnaea stagnalis exposed to i) dissolved MC-LR (33 and 100 μg L-1) and ii) Planktothrix agardhii suspensions producing 5 and 33 μg MC-LR equivalents L-1 over a 5-week period, and after a 3-week depuration period. Snails exposed to dissolved MC-LR accumulated up to 0.26 μg total MCs g-1 dry weight (DW), with no detection of bound MCs. Snails exposed to MCs producing P. agardhii accumulated up to 69.9 μg total MCs g-1 DW, of which from 17.7 to 66.7% were bound. After depuration, up to 15.3 μg g-1 DW of bound MCs were detected in snails previously exposed to toxic cyanobacteria, representing a potential source of MCs transfer through the food web.
AB - Accumulation of free microcystins (MCs) in freshwater gastropods has been demonstrated but accumulation of MCs covalently bound to tissues has never been considered so far. Here, we follow the accumulation of total (free and bound) MCs in Lymnaea stagnalis exposed to i) dissolved MC-LR (33 and 100 μg L-1) and ii) Planktothrix agardhii suspensions producing 5 and 33 μg MC-LR equivalents L-1 over a 5-week period, and after a 3-week depuration period. Snails exposed to dissolved MC-LR accumulated up to 0.26 μg total MCs g-1 dry weight (DW), with no detection of bound MCs. Snails exposed to MCs producing P. agardhii accumulated up to 69.9 μg total MCs g-1 DW, of which from 17.7 to 66.7% were bound. After depuration, up to 15.3 μg g-1 DW of bound MCs were detected in snails previously exposed to toxic cyanobacteria, representing a potential source of MCs transfer through the food web.
KW - Accumulation
KW - Covalently bound microcystins
KW - Cyanobacteria
KW - Detoxification
KW - Gastropods
UR - http://www.scopus.com/inward/record.url?scp=74149087519&partnerID=8YFLogxK
U2 - 10.1016/j.envpol.2009.10.025
DO - 10.1016/j.envpol.2009.10.025
M3 - Article
C2 - 19906474
AN - SCOPUS:74149087519
SN - 0269-7491
VL - 158
SP - 674
EP - 680
JO - Environmental Pollution
JF - Environmental Pollution
IS - 3
ER -