A time-resolved fluoroimmunometric assay for the detection of microcystins, cyanobacterial peptide hepatotoxins

Mehto; Ankelo; Hinkkanen; Mikhailov; John Eriksson; Lisa Spoof; Jussi Meriluoto

A time-resolved fluoroimmunometric assay for the detection of microcystins, cyanobacterial peptide hepatotoxins

Mehto
, Ankelo
, Hinkkanen
, Mikhailov
, John Eriksson
, Lisa Spoof
, Jussi Meriluoto

Research output: Contribution to journal › Article › Scientific › peer-review

26 Citations (Scopus)

Abstract

An immunoassay based on the time-resolved fluorometry (TR-FIA) was developed for microcystins, cyanobacterial peptide hepatotoxins. The assay was performed in a competitive mode and it utilised the monoclonal antibodies raised against microcystin-LR, and a europium chelate of microcystin-LR as a competitive antigen. The sensitivity of the assay was 0.1microg/l. The detection method of TR-FIA was compared to a commercially available kit based on the enzyme-linked immunosorbent assay (ELISA). The same level of sensitivity could be obtained with TR-FIA (in a non-optimised system). The simplified method of TR-FIA leads to a shorter analysis time.

Original language	Undefined/Unknown
Pages (from-to)	831–836
Journal	Toxicon
Volume	39
Issue number	6
Publication status	Published - 2001
MoE publication type	A1 Journal article-refereed

Cite this

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title = "A time-resolved fluoroimmunometric assay for the detection of microcystins, cyanobacterial peptide hepatotoxins",

abstract = "An immunoassay based on the time-resolved fluorometry (TR-FIA) was developed for microcystins, cyanobacterial peptide hepatotoxins. The assay was performed in a competitive mode and it utilised the monoclonal antibodies raised against microcystin-LR, and a europium chelate of microcystin-LR as a competitive antigen. The sensitivity of the assay was 0.1microg/l. The detection method of TR-FIA was compared to a commercially available kit based on the enzyme-linked immunosorbent assay (ELISA). The same level of sensitivity could be obtained with TR-FIA (in a non-optimised system). The simplified method of TR-FIA leads to a shorter analysis time.",

author = "Mehto and Ankelo and Hinkkanen and Mikhailov and John Eriksson and Lisa Spoof and Jussi Meriluoto",

year = "2001",

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volume = "39",

pages = "831–836",

journal = "Toxicon",

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TY - JOUR

T1 - A time-resolved fluoroimmunometric assay for the detection of microcystins, cyanobacterial peptide hepatotoxins

AU - Mehto, null

AU - Ankelo, null

AU - Hinkkanen, null

AU - Mikhailov, null

AU - Eriksson, John

AU - Spoof, Lisa

AU - Meriluoto, Jussi

PY - 2001

Y1 - 2001

N2 - An immunoassay based on the time-resolved fluorometry (TR-FIA) was developed for microcystins, cyanobacterial peptide hepatotoxins. The assay was performed in a competitive mode and it utilised the monoclonal antibodies raised against microcystin-LR, and a europium chelate of microcystin-LR as a competitive antigen. The sensitivity of the assay was 0.1microg/l. The detection method of TR-FIA was compared to a commercially available kit based on the enzyme-linked immunosorbent assay (ELISA). The same level of sensitivity could be obtained with TR-FIA (in a non-optimised system). The simplified method of TR-FIA leads to a shorter analysis time.

AB - An immunoassay based on the time-resolved fluorometry (TR-FIA) was developed for microcystins, cyanobacterial peptide hepatotoxins. The assay was performed in a competitive mode and it utilised the monoclonal antibodies raised against microcystin-LR, and a europium chelate of microcystin-LR as a competitive antigen. The sensitivity of the assay was 0.1microg/l. The detection method of TR-FIA was compared to a commercially available kit based on the enzyme-linked immunosorbent assay (ELISA). The same level of sensitivity could be obtained with TR-FIA (in a non-optimised system). The simplified method of TR-FIA leads to a shorter analysis time.

M3 - Artikel

SN - 0041-0101

VL - 39

SP - 831

EP - 836

JO - Toxicon

JF - Toxicon

IS - 6

ER -