A time-resolved fluoroimmunometric assay for the detection of microcystins, cyanobacterial peptide hepatotoxins

Mehto, Ankelo, Hinkkanen, Mikhailov, John Eriksson, Lisa Spoof, Jussi Meriluoto

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    Abstract

    An immunoassay based on the time-resolved fluorometry (TR-FIA) was developed for microcystins, cyanobacterial peptide hepatotoxins. The assay was performed in a competitive mode and it utilised the monoclonal antibodies raised against microcystin-LR, and a europium chelate of microcystin-LR as a competitive antigen. The sensitivity of the assay was 0.1microg/l. The detection method of TR-FIA was compared to a commercially available kit based on the enzyme-linked immunosorbent assay (ELISA). The same level of sensitivity could be obtained with TR-FIA (in a non-optimised system). The simplified method of TR-FIA leads to a shorter analysis time.
    Original languageUndefined/Unknown
    Pages (from-to)831–836
    JournalToxicon
    Volume39
    Issue number6
    Publication statusPublished - 2001
    MoE publication typeA1 Journal article-refereed

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