Abstract
An immunoassay based on the time-resolved fluorometry (TR-FIA) was developed for microcystins, cyanobacterial peptide hepatotoxins. The assay was performed in a competitive mode and it utilised the monoclonal antibodies raised against microcystin-LR, and a europium chelate of microcystin-LR as a competitive antigen. The sensitivity of the assay was 0.1microg/l. The detection method of TR-FIA was compared to a commercially available kit based on the enzyme-linked immunosorbent assay (ELISA). The same level of sensitivity could be obtained with TR-FIA (in a non-optimised system). The simplified method of TR-FIA leads to a shorter analysis time.
Original language | Undefined/Unknown |
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Pages (from-to) | 831–836 |
Journal | Toxicon |
Volume | 39 |
Issue number | 6 |
Publication status | Published - 2001 |
MoE publication type | A1 Journal article-refereed |