A novel solid-state electrochemiluminescence sensor for detection of cytochrome c based on ceria nanoparticles decorated with reduced graphene oxide nanocomposite

Mohammad Reza Karimi Pur; Morteza Hosseini; Farnoush Faridbod; Amin Shiralizadeh Dezfuli; Mohammad Reza Ganjali

doi:10.1007/s00216-016-9856-6

A novel solid-state electrochemiluminescence sensor for detection of cytochrome c based on ceria nanoparticles decorated with reduced graphene oxide nanocomposite

Mohammad Reza Karimi Pur, Morteza Hosseini^*, Farnoush Faridbod, Amin Shiralizadeh Dezfuli, Mohammad Reza Ganjali

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

58 Citations (Scopus)

Abstract

A novel ultrasensitive sensing system for the rapid detection of cytochrome c (Cyt C) was developed on the basis of an electrochemiluminescence (ECL) method. A nanocomposite biosensor was made of reduced graphene oxide decorated with cerium oxide/tris(2,2-bipyridyl)ruthenium(II)/chitosan (CeO₂NPs-RGO/ Ru(bpy)₃ ²⁺/CHIT) and used for this purpose. The ECL signal was produced by an electrochemical interaction between Ru(bpy)₃ ²⁺ and tripropyl amine (TPA) on the surface of the electrode. Addition of Cyt C to the solution decreases the ECL signal due to its affinity for TPA and inhibition of its reaction with Ru(bpy)₃ ²⁺. The effects of the amount of CeO₂NPs-RGO, Ru(bpy)₃ ²⁺, TPA concentration as a co-reactant, and the pH of the electrolyte solution on the ECL signal intensity were studied and optimized. The results showed that the method was fast, reproducible, sensitive, and stable for the detection of Cyt C. The method has a linear range from 2.5 nM to 2 μM (R² = 0.995) with a detection limit of 0.7 nM. Finally, the proposed biosensor was used for the determination of Cyt C in human serum samples with RSDs of 1.8–3.6 %. The results demonstrate that this solid-state ECL quenching biosensor has high sensitivity, selectivity, and good stability. [Figure not available: see fulltext.]

Original language	English
Pages (from-to)	7193-7202
Number of pages	10
Journal	Analytical and Bioanalytical Chemistry
Volume	408
Issue number	25
DOIs	https://doi.org/10.1007/s00216-016-9856-6
Publication status	Published - 1 Oct 2016
Externally published	Yes
MoE publication type	A1 Journal article-refereed

Funding

The authors thank the Research Council of University of Tehran for financial support of this work.

Keywords

Biosensor
Cerium oxide
Electrochemiluminescence
Reduced graphene

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10.1007/s00216-016-9856-6Licence: All rights reserved

Cite this

@article{37d2814aec6a4771a5b4e17150020c10,

title = "A novel solid-state electrochemiluminescence sensor for detection of cytochrome c based on ceria nanoparticles decorated with reduced graphene oxide nanocomposite",

abstract = "A novel ultrasensitive sensing system for the rapid detection of cytochrome c (Cyt C) was developed on the basis of an electrochemiluminescence (ECL) method. A nanocomposite biosensor was made of reduced graphene oxide decorated with cerium oxide/tris(2,2-bipyridyl)ruthenium(II)/chitosan (CeO2NPs-RGO/ Ru(bpy)3 2+/CHIT) and used for this purpose. The ECL signal was produced by an electrochemical interaction between Ru(bpy)3 2+ and tripropyl amine (TPA) on the surface of the electrode. Addition of Cyt C to the solution decreases the ECL signal due to its affinity for TPA and inhibition of its reaction with Ru(bpy)3 2+. The effects of the amount of CeO2NPs-RGO, Ru(bpy)3 2+, TPA concentration as a co-reactant, and the pH of the electrolyte solution on the ECL signal intensity were studied and optimized. The results showed that the method was fast, reproducible, sensitive, and stable for the detection of Cyt C. The method has a linear range from 2.5 nM to 2 μM (R2 = 0.995) with a detection limit of 0.7 nM. Finally, the proposed biosensor was used for the determination of Cyt C in human serum samples with RSDs of 1.8–3.6 \%. The results demonstrate that this solid-state ECL quenching biosensor has high sensitivity, selectivity, and good stability. [Figure not available: see fulltext.]",

keywords = "Biosensor, Cerium oxide, Electrochemiluminescence, Reduced graphene",

author = "Pur, \{Mohammad Reza Karimi\} and Morteza Hosseini and Farnoush Faridbod and Dezfuli, \{Amin Shiralizadeh\} and Ganjali, \{Mohammad Reza\}",

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doi = "10.1007/s00216-016-9856-6",

language = "English",

volume = "408",

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A novel solid-state electrochemiluminescence sensor for detection of cytochrome c based on ceria nanoparticles decorated with reduced graphene oxide nanocomposite. / Pur, Mohammad Reza Karimi; Hosseini, Morteza; Faridbod, Farnoush et al.
In: Analytical and Bioanalytical Chemistry, Vol. 408, No. 25, 01.10.2016, p. 7193-7202.

Research output: Contribution to journal › Article › Scientific › peer-review

TY - JOUR

T1 - A novel solid-state electrochemiluminescence sensor for detection of cytochrome c based on ceria nanoparticles decorated with reduced graphene oxide nanocomposite

AU - Pur, Mohammad Reza Karimi

AU - Hosseini, Morteza

AU - Faridbod, Farnoush

AU - Dezfuli, Amin Shiralizadeh

AU - Ganjali, Mohammad Reza

PY - 2016/10/1

Y1 - 2016/10/1

N2 - A novel ultrasensitive sensing system for the rapid detection of cytochrome c (Cyt C) was developed on the basis of an electrochemiluminescence (ECL) method. A nanocomposite biosensor was made of reduced graphene oxide decorated with cerium oxide/tris(2,2-bipyridyl)ruthenium(II)/chitosan (CeO2NPs-RGO/ Ru(bpy)3 2+/CHIT) and used for this purpose. The ECL signal was produced by an electrochemical interaction between Ru(bpy)3 2+ and tripropyl amine (TPA) on the surface of the electrode. Addition of Cyt C to the solution decreases the ECL signal due to its affinity for TPA and inhibition of its reaction with Ru(bpy)3 2+. The effects of the amount of CeO2NPs-RGO, Ru(bpy)3 2+, TPA concentration as a co-reactant, and the pH of the electrolyte solution on the ECL signal intensity were studied and optimized. The results showed that the method was fast, reproducible, sensitive, and stable for the detection of Cyt C. The method has a linear range from 2.5 nM to 2 μM (R2 = 0.995) with a detection limit of 0.7 nM. Finally, the proposed biosensor was used for the determination of Cyt C in human serum samples with RSDs of 1.8–3.6 %. The results demonstrate that this solid-state ECL quenching biosensor has high sensitivity, selectivity, and good stability. [Figure not available: see fulltext.]

AB - A novel ultrasensitive sensing system for the rapid detection of cytochrome c (Cyt C) was developed on the basis of an electrochemiluminescence (ECL) method. A nanocomposite biosensor was made of reduced graphene oxide decorated with cerium oxide/tris(2,2-bipyridyl)ruthenium(II)/chitosan (CeO2NPs-RGO/ Ru(bpy)3 2+/CHIT) and used for this purpose. The ECL signal was produced by an electrochemical interaction between Ru(bpy)3 2+ and tripropyl amine (TPA) on the surface of the electrode. Addition of Cyt C to the solution decreases the ECL signal due to its affinity for TPA and inhibition of its reaction with Ru(bpy)3 2+. The effects of the amount of CeO2NPs-RGO, Ru(bpy)3 2+, TPA concentration as a co-reactant, and the pH of the electrolyte solution on the ECL signal intensity were studied and optimized. The results showed that the method was fast, reproducible, sensitive, and stable for the detection of Cyt C. The method has a linear range from 2.5 nM to 2 μM (R2 = 0.995) with a detection limit of 0.7 nM. Finally, the proposed biosensor was used for the determination of Cyt C in human serum samples with RSDs of 1.8–3.6 %. The results demonstrate that this solid-state ECL quenching biosensor has high sensitivity, selectivity, and good stability. [Figure not available: see fulltext.]

KW - Biosensor

KW - Cerium oxide

KW - Electrochemiluminescence

KW - Reduced graphene

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M3 - Article

C2 - 27558103

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SN - 1618-2642

VL - 408

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JO - Analytical and Bioanalytical Chemistry

JF - Analytical and Bioanalytical Chemistry

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