A jet ring cell with a renewable solid support for amperometric detection of glucose in a sequential injection analysis system

Tom Lindfors, Ilkka Lähdesmäki, Ari Ivaska

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    A new amperometric method for the detection of glucose has been developed. A jet ring cell with a renewable solid support (JRR) is connected to a sequential injection analysis (SIA) system. The solid support, consisting of small spherical particles of agarose gel, with a mean bead size of 90 mu m, is coupled with the enzyme glucose oxidase (GOD). The suspension is injected through a multiport valve in the SIA-system and is trapped in the JRR cell by a gap of 10 mu m between the glassy carbon (GC) working electrode and a stainless steel counter electrode. The subsequent injection of glucose results in formation of hydrogen peroxide, which is detected by oxidation at 1.0 V (vs. Ag/AgCl/KCl (3 M)), Thereafter the suspension is removed by reversing the pump flow and subsequently replaced with fresh suspension for the next determination of glucose, The Linear working range for the JRR-sensor, at this stage of the work, is 100 mu M - 5 mM.
    Original languageUndefined/Unknown
    Pages (from-to)2257–2267
    Number of pages11
    JournalAnalytical Letters
    Issue number13
    Publication statusPublished - 1996
    MoE publication typeA1 Journal article-refereed


    • glucose
    • renewable solid support
    • sequential injection analysis

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