α5β1 integrin recycling promotes Arp2/3-independent cancer cell invasion via the formin FHOD3.

Nikki R. Paul, Jennifer L. Allen, Anna Chapman, Maria Morlan-Mairal, Egor Zindy, Guillaume Jacquemet, Laura Fernandez del Ama, Nermina Ferizovic, David M. Green, Jonathan D. Howe, Elisabeth Ehler, Adam Hurlstone, Patrick T. Caswell

Research output: Contribution to journalArticleScientificpeer-review

60 Citations (Scopus)

Abstract

Invasive migration in 3D extracellular matrix (ECM) is crucial to cancer metastasis, yet little is known of the molecular mechanisms that drive reorganization of the cytoskeleton as cancer cells disseminate in vivo. 2D Rac-driven lamellipodial migration is well understood, but how these features apply to 3D migration is not clear. We find that lamellipodia-like protrusions and retrograde actin flow are indeed observed in cells moving in 3D ECM. However, Rab-coupling protein (RCP)-driven endocytic recycling of α5β1 integrin enhances invasive migration of cancer cells into fibronectin-rich 3D ECM, driven by RhoA and filopodial spike-based protrusions, not lamellipodia. Furthermore, we show that actin spike protrusions are Arp2/3-independent. Dynamic actin spike assembly in cells invading in vitro and in vivo is regulated by Formin homology-2 domain containing 3 (FHOD3), which is activated by RhoA/ROCK, establishing a novel mechanism through which the RCP-α5β1 pathway reprograms the actin cytoskeleton to promote invasive migration and local invasion in vivo.
Original languageUndefined/Unknown
Pages (from-to)1013–1031
JournalJournal of Cell Biology
Volume210
Issue number6
DOIs
Publication statusPublished - 2015
MoE publication typeA1 Journal article-refereed

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