Purification and Validation of Lipid Transfer Proteins

A3 Bokavsnitt, kapitel i forskningsböcker


Interna författare/redaktörer


Publikationens författare: Matti A. Kjellberg, Anders P.E. Backman, Anna Möuts, Peter Mattjus
Redaktörer: Sanjoy K. Bhattacharya
Publiceringsår: 2017
Förläggare: Humana Press
Moderpublikationens namn: Lipidomics - Methods and Protocols
Seriens namn: Methods in Molecular Biology
Volym: 1609
ISBN: 978-1-4939-6995-1


Abstrakt

Understanding the holistic picture of lipid homeostasis involves not only the analysis of synthesis and breakdown of lipids, but also requires a thorough understanding of their transport. The transport of lipid monomers in an aqueous environment is facilitated by different lipid transfer proteins. Their universal feature is the shielding or encapsulation of the hydrophobic part of the lipid, consequently overcoming the poor solubility of lipids in water. Here we describe a method to purify lipid transfer proteins using bacterial expression. We also present three methods to validate their transfer activity.


Nyckelord

Fluorescence, lipid, surface plasmon resonance, vesicle

Senast uppdaterad 2019-19-09 vid 04:51