Evolutionary History of the Non-Specific Lipid Transfer Proteins

A1 Originalartikel i en vetenskaplig tidskrift (referentgranskad)


Interna författare/redaktörer


Publikationens författare: Edstam MM, Viitanen L, Salminen TA, Edqvist J
Förläggare: OXFORD UNIV PRESS
Publiceringsår: 2011
Tidskrift: Molecular Plant
Tidskriftsakronym: MOL PLANT
Volym: 4
Nummer: 6
Artikelns första sida, sidnummer: 947
Artikelns sista sida, sidnummer: 964
Antal sidor: 18
ISSN: 1674-2052


Abstrakt

The non-specific lipid transfer proteins (nsLTPs) are small, basic proteins characterized by a tunnel-like hydrophobic cavity, capable of transferring various lipid molecules between lipid bilayers. Most nsLTPs are synthesized with an N-terminal signal peptide that localizes the protein to the apoplastic space. The nsLTPs have only been identified in seed plants, where they are encoded by large gene families. We have initiated an analysis of the evolutionary history of the nsLTP family using genomic and EST information from non-seed land plants and green algae to determine: (1) when the nsLTP family arose, (2) how often new nsLTP subfamilies have been created, and (3) how subfamilies differ in their patterns of expansion and loss in different plant lineages. In this study, we searched sequence databases and found that genes and transcripts encoding nsLTPs are abundant in liverworts, mosses, and all other investigated land plants, but not present in any algae. The tertiary structures of representative liverwort and moss nsLTPs were further studied with homology modeling. The results indicate that the nsLTP family has evolved after plants conquered land. Only two of the four major subfamilies of nsLTPs found in flowering plants are present in mosses and liverworts. The additional subfamilies have arisen later, during land plant evolution. In this report, we also introduce a modified nsLTP classification system.


Nyckelord

bryophytes, evolutionary genetics, molecular biology, molecular evolution

Senast uppdaterad 2019-18-10 vid 04:36