The L1TD1 protein interactome reveals the importance of post-transcriptional regulation in human pluripotency

A1 Originalartikel i en vetenskaplig tidskrift (referentgranskad)


Interna författare/redaktörer


Publikationens författare: Emani MR, Närvä E, Stubb A, Chakroborty D, Viitala M, Rokka A, Rahkonen N, Moulder R, Denessiouk K, Trokovic R, Lund R, Elo LL, Lahesmaa R.
Förläggare: Elsevier
Publiceringsår: 2015
Tidskrift: Stem Cell Reports
Tidskriftsakronym: Stem Cell Reports
Volym: 4
Nummer: 3
Artikelns första sida, sidnummer: 519
Artikelns sista sida, sidnummer: 528
eISSN: 2213-6711


Abstrakt

The RNA-binding protein L1TD1 is one of the most specific and abundant proteins in pluripotent stem cells and is essential for the maintenance of pluripotency in human cells. Here, we identify the protein interaction network of L1TD1 in human embryonic stem cells (hESCs) and provide insights into the interactome network constructed in human pluripotent cells. Our data reveal that L1TD1 has an important role in RNA splicing, translation, protein traffic, and degradation. L1TD1 interacts with multiple stem-cell-specific proteins, many of which are still uncharacterized in the context of development. Further, we show that L1TD1 is a part of the pluripotency interactome network of OCT4, SOX2, and NANOG, bridging nuclear and cytoplasmic regulation and highlighting the importance of RNA biology in pluripotency.


Nyckelord

Protein Interactome


Dokument


Senast uppdaterad 2019-21-10 vid 03:22