The L1TD1 protein interactome reveals the importance of post-transcriptional regulation in human pluripotency

A1 Journal article (refereed)


Internal Authors/Editors


Publication Details

List of Authors: Emani MR, Närvä E, Stubb A, Chakroborty D, Viitala M, Rokka A, Rahkonen N, Moulder R, Denessiouk K, Trokovic R, Lund R, Elo LL, Lahesmaa R.
Publisher: Elsevier
Publication year: 2015
Journal: Stem Cell Reports
Journal acronym: Stem Cell Reports
Volume number: 4
Issue number: 3
Start page: 519
End page: 528
eISSN: 2213-6711


Abstract

The RNA-binding protein L1TD1 is one of the most specific and abundant proteins in pluripotent stem cells and is essential for the maintenance of pluripotency in human cells. Here, we identify the protein interaction network of L1TD1 in human embryonic stem cells (hESCs) and provide insights into the interactome network constructed in human pluripotent cells. Our data reveal that L1TD1 has an important role in RNA splicing, translation, protein traffic, and degradation. L1TD1 interacts with multiple stem-cell-specific proteins, many of which are still uncharacterized in the context of development. Further, we show that L1TD1 is a part of the pluripotency interactome network of OCT4, SOX2, and NANOG, bridging nuclear and cytoplasmic regulation and highlighting the importance of RNA biology in pluripotency.


Keywords

Protein Interactome


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