Solution Conformation of Polymer Brushes Determines Their Interactions with DNA and Transfection Efficiency

A1 Journal article (refereed)


Internal Authors/Editors


Publication Details

List of Authors: Krishnamoorthy M, Li D, Sharili AS, Gulin-Sarfraz T, Rosenholm JM, Gautrot JE
Publisher: American Chemical Society
Publication year: 2017
Journal: Biomacromolecules
Volume number: 18
Issue number: 12
Start page: 4121
End page: 4132
ISSN: 1525-7797


Abstract

Polymer brush-functionalized nanomaterials offer interesting features
for the design of gene delivery vectors as their physicochemical and
structural properties can be designed independently of the chemistry,
size and shape of the nanomaterial core. However, little is known of the
parameters regulating the adsorption and infiltration of DNA molecules
at the surface of positively charged polymer brushes, despite the
importance of such processes for gene delivery. Here we investigate the
role of the molecular environment (e.g., pH, type of buffer,
concentration) on the interactions between plasmid DNA and positively
charged poly(dimethylaminoethyl methacrylate) (PDMAEMA) brushes using a
combination of light scattering, electrophoretic light scattering, in
situ ellipsometry, and surface plasmon resonance. We show that the
conformation of swollen PDMAEMA brushes is modulated by the surrounding
buffer and that this impacts strongly on the ability of such brushes and
nanomaterials based on these coatings to complex DNA molecules. In
turn, the levels of transfection efficiency measured correlate with
changes in brush conformation and DNA binding. Therefore, this work
demonstrates the importance of molecular design of polymer brushes to
control DNA complexation and release in order to optimize the
performance of polymer brush-functionalized nanomaterials for gene
delivery applications.


Last updated on 2019-22-10 at 04:49