Printed paper-based arrays as substrates for biofilm formation

A1 Journal article (refereed)

Internal Authors/Editors

Publication Details

List of Authors: Anni Määttänen, Adyary Fallarero, Janni Kujala, Petri Ihalainen, Pia Vuorela, Jouko Peltonen
Publication year: 2014
Journal: AMB Express
Volume number: 4
Issue number: 1
Start page: 1
End page: 12
ISSN: 2191-0855


The suitability of paper-based arrays for biofilm formation studies by Staphylococcus aureus is demonstrated. Laboratory-coated papers with different physicochemical properties were used as substrates. The array platform was fabricated by patterning the coated papers with vinyl-substituted polydimethylsiloxane (PDMS) -based ink. The affinity of bacteria onto the flexographically printed hydrophobic and smooth PDMS film was very low whereas bacterial adhesion and biofilm formation occurred preferentially on the unprinted areas, i.e. in the reaction arrays. The concentration of the attached bacteria was quantified by determining the viable colony forming unit (CFU/cm2) numbers. The distribution and the extent of surface coverage of the biofilms were determined by atomic force microscopy. In static conditions, the highest bacterial concentration and most highly organized biofilms were observed on substrates with high polarity. On a rough paper surface with low polarity, the biofilm formation was most hindered. Biofilms were effectively removed from a polar substrate upon exposure to (+)-dehydroabietic acid, an anti-biofilm compound.


Anti-biofilm activity, atomic force microscopy (AFM), Biofilm, printing, surface chemistry, surface roughness


Last updated on 2020-25-02 at 05:43