Transcriptional response to stress is pre-wired by promoter and enhancer architecture

A1 Journal article (refereed)


Internal Authors/Editors


Publication Details

List of Authors: Vihervaara A, Mahat DB, Guertin MJ, Chu TY, Danko CG, Lis JT, Sistonen L
Publisher: NATURE PUBLISHING GROUP
Publication year: 2017
Journal: Nature Communications
Journal acronym: NAT COMMUN
Volume number: 8
Number of pages: 16
ISSN: 2041-1723
eISSN: 2041-1723


Abstract

Programs of gene expression are executed by a battery of transcription factors that coordinate divergent transcription from a pair of tightly linked core initiation regions of promoters and enhancers. Here, to investigate how divergent transcription is reprogrammed upon stress, we measured nascent RNA synthesis at nucleotide-resolution, and profiled histone H4 acetylation in human cells. Our results globally show that the release of promoter-proximal paused RNA polymerase into elongation functions as a critical switch at which a gene's response to stress is determined. Highly transcribed and highly inducible genes display strong transcriptional directionality and selective assembly of general transcription factors on the core sense promoter. Heat-induced transcription at enhancers, instead, correlates with prior binding of cell-type, sequence-specific transcription factors. Activated Heat Shock Factor 1 (HSF1) binds to transcription-primed promoters and enhancers, and CTCF-occupied, non-transcribed chromatin. These results reveal chromatin architectural features that orient transcription at divergent regulatory elements and prime transcriptional responses genome-wide.

Last updated on 2019-21-05 at 05:38

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