Hybrid mesoporous nanorods with deeply grooved lateral faces toward cytosolic drug delivery

A1 Originalartikel i en vetenskaplig tidskrift (referentgranskad)


Interna författare/redaktörer


Publikationens författare: Sun K, Ding T, Xing Y, Mo D, Zhang J, Rosenholm JM
Förläggare: Royal Society of Chemistry
Publiceringsår: 2019
Tidskrift: Biomaterials Science
Volym: 7
Nummer: 12
Artikelns första sida, sidnummer: 5301
Artikelns sista sida, sidnummer: 5311


Abstrakt

Nanocarriers with high local curvatures hold a great potential of
inducing effective penetration of intracellular barriers and cytosolic
delivery of membrane-impermeable drugs. However, the fine control of the
sharp edges and their morphological effects inside cells remains
largely unexplored. Herein, a nanocarrier system of hybrid mesoporous
nanorods with six-arm star-shaped end faces and groove-patterned lateral
faces was developed to maximize surface regions with high local
curvatures for enhancing membrane destabilization. Specifically, twisted
(right-handed) nanorods (TNR, diameter ∼120, aspect ratio 4–5) with a
hexagon cross-section from a templated synthesis were modified by amino
groups to promote surface coating of a wet-adhesive polymer, i.e.
polydopamine (PDA). An edge-preferential deposition of PDA by local
curvature effects led to the protective etching of silica, and in turn,
the formation of nanorods with varying groove depths at different
volumes of the aqueous coating solution. Finally, branched polyethylene
imine (PEI) was grafted on the exterior surface of the nanorods for
enhancing the dispersity and cellular uptake rate. As verified by
elaborate in vitro investigations, the configuration of
nanorods with the sharpest edges/deepest grooves can be rotated to a
lying-down/upright mode in order to minimize/maximize the membrane
tension during the interaction with membranes, which consequently
resulted in highly efficient lysosomal escape despite the relatively
lower uptake degree. The successful delivery of vorinostat (SAHA, a
FDA-approved histone deacetylase inhibitor) and inhibition of cancer
cells demonstrated the attractive ability of the nanocarriers in drug
delivery.


Nyckelord

controlled drug delivery, drug delivery, drug-delivery systems, intracellular transport, mesoporous materials, Mesoporous silica, nanorods


Dokument


Senast uppdaterad 2020-28-02 vid 04:33