Filopodia Quantification Using FiloQuant.

A3 Bokavsnitt, kapitel i forskningsböcker


Interna författare/redaktörer


Publikationens författare: Jacquemet, Hamidi, Ivaska
Redaktörer: Elena Rebollo, Manel Bosch
Publiceringsår: 2019
Tidskrift: Methods in Molecular Biology
Förläggare: Humana Press
Moderpublikationens namn: Computer Optimized Microscopy
Tidskriftsakronym: Methods Mol Biol
Seriens namn: Methods in Molecular Biology
Volym: 2040
Artikelns första sida, sidnummer: 359
Artikelns sista sida, sidnummer: 373
ISBN: 978-1-4939-9685-8
ISSN: 1064-3745


Abstrakt

Filopodia are fingerlike membrane protrusions that are extended by cells in vitro and in vivo. Due to important roles in sensing the extracellular microenvironment, filopodia and filopodia-like protrusions have been implicated in numerous biological processes including epithelial sheet zippering in development and wound healing and in cancer progression. Recently, there has been an explosion in the number of software available to analyze specific features of cell protrusions with the aim of gaining mechanistic insights into the action of filopodia and filopodia-like structures. In this methods chapter, we highlight an open-access software called FiloQuant that has been developed to specifically quantify the length, density, and dynamics of filopodia and filopodia-like structures from in vitro and in vivo generated samples. We provide step-by-step protocols on (i) how to install FiloQuant in the ImageJ platform (Fiji), (ii) how to quantify filopodia and filopodia-like protrusions from single images using FiloQuant, and (iii) how to track filopodial protrusions from live-cell imaging experiments using FiloQuant and TrackMate.

Senast uppdaterad 2020-24-02 vid 03:03