Filopodia Quantification Using FiloQuant.

A1 Journal article (refereed)


Internal Authors/Editors


Publication Details

List of Authors: Jacquemet, Hamidi, Ivaska
Publication year: 2019
Journal: Methods in Molecular Biology
Journal acronym: Methods Mol Biol
Volume number: 2040
Start page: 359
End page: 373
ISSN: 1940-6029


Abstract

Filopodia are fingerlike membrane protrusions that are extended by cells in vitro and in vivo. Due to important roles in sensing the extracellular microenvironment, filopodia and filopodia-like protrusions have been implicated in numerous biological processes including epithelial sheet zippering in development and wound healing and in cancer progression. Recently, there has been an explosion in the number of software available to analyze specific features of cell protrusions with the aim of gaining mechanistic insights into the action of filopodia and filopodia-like structures. In this methods chapter, we highlight an open-access software called FiloQuant that has been developed to specifically quantify the length, density, and dynamics of filopodia and filopodia-like structures from in vitro and in vivo generated samples. We provide step-by-step protocols on (i) how to install FiloQuant in the ImageJ platform (Fiji), (ii) how to quantify filopodia and filopodia-like protrusions from single images using FiloQuant, and (iii) how to track filopodial protrusions from live-cell imaging experiments using FiloQuant and TrackMate.

Last updated on 2019-12-11 at 03:22