Bioactive glass ions induce efficient osteogenic differentiation of human adipose stem cells encapsulated in gellan gum and collagen type I hydrogels

A1 Journal article (refereed)

Internal Authors/Editors

Publication Details

List of Authors: Kaisa Vuornos, Miina Ojansivu, Janne T Koivisto, Heikki Häkkänen, Birhanu Belay, Toni Montonen, Heini Huhtala, Minna Kääriäinen, Leena Hupa, Minna Kellomäki, Jari Hyttinen, Janne A Ihalainen, Susanna Miettinen
Publisher: Elsevier
Publication year: 2019
Journal: Materials Science and Engineering: C
Volume number: 99
Start page: 905
End page: 918



to unmet need for bone augmentation, our aim was to promote osteogenic
differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2
50.0) extract based osteogenic medium (BaG OM) for bone construct
development. GG hydrogels were crosslinked with spermidine (GG-SPD) or
BaG extract (GG-BaG).


properties of cell-free GG-SPD, GG-BaG, and COL hydrogels were tested
in osteogenic medium (OM) or BaG OM at 0, 14, and 21 d. Hydrogel
embedded hASCs were cultured in OM or BaG OM for 3, 14, and 21 d, and
analyzed for viability, cell number, osteogenic gene expression,
osteocalcin production, and mineralization. Hydroxyapatite-stained
GG-SPD samples were imaged with Optical Projection Tomography (OPT) and Selective Plane Illumination Microscopy (SPIM) in OM and BaG OM at 21 d. Furthermore, Raman spectroscopy was used to study the calcium phosphate (CaP) content of hASC-secreted ECM in GG-SPD, GG-BaG, and COL at 21 d in BaG OM.


results showed viable rounded cells in GG whereas hASCs were elongated
in COL. Importantly, BaG OM induced significantly higher cell number and
higher osteogenic gene expression in COL. In both hydrogels, BaG OM
induced strong mineralization confirmed as CaP by Raman spectroscopy and
significantly improved mechanical properties. GG-BaG hydrogels rescued
hASC mineralization in OM. OPT and SPIM showed homogeneous 3D cell
distribution with strong mineralization in BaG OM. Also, strong
osteocalcin production was visible in COL.


Overall, we showed efficacious osteogenesis of hASCs in 3D hydrogels with BaG OM with potential for bone-like grafts.

Last updated on 2020-03-04 at 09:16