EFFECTS OF DEHYDROABIETIC ACID ON THE ERYTHROCYTE MEMBRANE

A1 Journal article (refereed)


Internal Authors/Editors


Publication Details

List of Authors: TOIVOLA DM, ISOMAA B
Publisher: ELSEVIER SCI IRELAND LTD
Publication year: 1991
Journal: Chemico-Biological Interactions
Journal acronym: CHEM-BIOL INTERACT
Volume number: 79
Issue number: 1
Start page: 65
End page: 78
Number of pages: 14
ISSN: 0009-2797


Abstract

The effects of dehydroabietic acid (DHAA), a dominant resin acid in pulp and paper mill effluents, on membrane-connected events were studied in human erythrocytes. Fifty percent haemolysis was achieved by 252-mu-M DHAA after 1 h of incubation at +37-degrees-C. At sublytic concentrations, DHAA protected erythrocytes against hypotonic haemolysis, with maximum protection occurring at 125-mu-M. In the lower range of sublytic concentrations, DHAA induced a slight echinocytosis; at higher sublytic concentrations erythrocytes were transformed to sphero-echinocytes and a release of acetylcholinesterase (exovesicles) occurred. Furthermore, at sublytic concentrations DHAA increased potassium efflux and passive potassium influx, while active potassium influx ((Na+-K+)-pump activity) and phosphate efflux were decreased. Our study indicates that DHAA acts on human erythrocytes in a way typical for amphiphilic compounds. It is proposed that DHAA by intercalating into the lipid bilayer of the membrane, affects the dynamics of the bilayer which in turn alters the permeability of the bilayer and the function of ion transporting membrane proteins.


Keywords

AMPHIPHILE, erythrocyte membrane, hemolysis, shape alteration

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