Analysis of the plasma proteome using iTRAQ and TMT-based Isobaric labeling

A2 Granskningsartikel, litteraturgranskning, systematisk granskning


Interna författare/redaktörer


Publikationens författare: Moulder R, Bhosale SD, Goodlett DR, Lahesmaa R
Förläggare: WILEY
Publiceringsår: 2018
Tidskrift: Mass Spectrometry Reviews
Tidskriftsakronym: MASS SPECTROM REV
Volym: 37
Nummer: 5
Artikelns första sida, sidnummer: 583
Artikelns sista sida, sidnummer: 606
Antal sidor: 24
ISSN: 0277-7037


Abstrakt

Over the past decade, chemical labeling with isobaric tandem mass tags, such as isobaric tags for relative and absolute quantification reagents (iTRAQ) and tandem mass tag (TMT) reagents, has been employed in a wide range of different clinically orientated serum and plasma proteomics studies. In this review the scope of these works is presented with attention to the areas of research, methods employed and performance limitations. These applications have covered a wide range of diseases, disorders and infections, and have implemented a variety of different preparative and mass spectrometric approaches. In contrast to earlier works, which struggled to quantify more than a few hundred proteins, increasingly these studies have provided deeper insight into the plasma proteome extending the numbers of quantified proteins to over a thousand.


Nyckelord

disease research, immunodepletion, isobaric labeling, peptide fractionation, protein biomarkers

Senast uppdaterad 2019-17-10 vid 04:41