Characterization of modified mesoporous silica nanoparticles as vectors for siRNA delivery

A1 Journal article (refereed)


Internal Authors/Editors


Publication Details

List of Authors: Slita A, Egorova A, Casals E, Kiselev A, Rosenholm JM
Publisher: Elsevier
Publication year: 2018
Journal: Asian Journal of Pharmaceutical Sciences
Volume number: 13
Issue number: 6
Start page: 592
End page: 599
ISSN: 1818-0876


Abstract

Gene therapy using siRNA
molecules is nowadays considered as a promising approach. For
successful therapy, development of a stable and reliable vector for
siRNA is crucial. Non-viral and non-organic vectors like mesoporous silica nanoparticles (MSN) are associated with lack of most viral vector drawbacks, such as toxicity, immunogenicity, but also generally a low nucleic acid
carrying capacity. To overcome this hurdle, we here modified the pore
walls of MSNs with surface-hyperbranching polymerized
poly(ethyleneimine) (hbPEI), which provides an abundance of amino-groups
for loading of a larger amount of siRNA molecules via electrostatic
adsorption. After loading, the particles were covered with a second
layer of pre-polymerized PEI to provide better protection of siRNA
inside the pores, more effective cellular uptake and endosomal escape.
To test the transfection efficiency of PEI covered siRNA/MSNs, MDA-MB
231 breast cancer cells stably expressing GFP were used. We demonstrate
that PEI-coated siRNA/MSN complexes provide more effective delivery of siRNAs
compared to unmodified MSNs. Thus, it can be concluded that
appropriately surface-modified MSNs can be considered as prospective
vectors for therapeutic siRNA delivery.


Keywords

Drug delivery, drug-delivery systems, Gene technology, mesoporous silica nanoparticles, Nanoparticles, siRNA


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Last updated on 2019-17-06 at 05:12