7-Hydroxymatairesinol improves body weight, fat and sugar metabolism in C57BJ/6 mice on a high-fat diet

A1 Journal article (refereed)


Internal Authors/Editors


Publication Details

List of Authors: Biasiotto G, Zanella I, Predolini F, Archetti I, Cadei M, Monti E, Luzzani M, Pacchetti B, Mozzoni P, Andreoli R, De Palma G, Serana F, Smeds A, Di Lorenzo D
Publisher: CAMBRIDGE UNIV PRESS
Publication year: 2018
Journal: British Journal of Nutrition
Journal acronym: BRIT J NUTR
Volume number: 120
Issue number: 7
Start page: 751
End page: 762
Number of pages: 12
ISSN: 0007-1145


Abstract

7-Hydroxymatairesinol (7-HMR) is a plant lignan abundant in various concentrations in plant foods. The objective of this study was to test HMRLignan (TM), a purified form of 7-HMR, and the corresponding Picea abies extract (total extract P. abies; TEP) as dietary supplements on a background of a high-fat diet (HFD)-induced metabolic syndrome in mice and in the 3T3-L1 adipogenesis model. Mice, 3 weeks old, were fed a HFD for 60 d. Subgroups were treated with 3 mg/kg body weight 7-HMR (HMRLignan (TM)) or 10 mg/kg body weight TEP by oral administration. 7-HMR and TEP limited the increase in body weight (-11 and -13%) and fat mass (-11 and -18%) in the HFD-fed mice. Epididymal adipocytes were 19 and -12 % smaller and the liver was less steatotic (-62 and -65%). Serum lipids decreased in TEP-treated mice (-11% cholesterol, -23 % LDL and -15 % TAG) and sugar metabolism was ameliorated by both lignan preparations, as shown by a more than 70% decrease in insulin secretion and insulin resistance. The expression of several metabolic genes was modulated by the HFD with an effect that was reversed by lignan. In 3T3-L1 cells, the 7-HMR metabolites enterolactone (ENL) and enterodiol (END) showed a 40 % inhibition of cell differentiation accompanied by the inhibited expression of the adipogenic genes PPAR gamma, C/EBP alpha and aP2. Furthermore, END and ENL caused a 10 % reduction in TAG uptake in HEPA 1-6 hepatoma cells. In conclusion, 7-HMR and TEP reduce metabolic imbalances typical of the metabolic syndrome and obesity in male mice, whereas their metabolites inhibit adipogenesis and lipid uptake in vitro.


Keywords

7-hydroxymatairesinol, Adipocyte differentiation, Adipose deposition


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Last updated on 2019-22-08 at 05:29