Significance of the transient receptor potential canonical 2 (TRPC2) channel in the regulation of rat thyroid FRTL-5 cell proliferation, migration, adhesion and invasion

A1 Originalartikel i en vetenskaplig tidskrift (referentgranskad)


Interna författare/redaktörer


Publikationens författare: Sukumaran P, Löf C, Pulli I, Kemppainen K, Viitanen T, Törnquist K
Förläggare: ELSEVIER IRELAND LTD
Publiceringsår: 2013
Tidskrift: Molecular and Cellular Endocrinology
Tidskriftsakronym: MOL CELL ENDOCRINOL
Volym: 374
Nummer: 1-2
Artikelns första sida, sidnummer: 10
Artikelns sista sida, sidnummer: 21
Antal sidor: 12
ISSN: 0303-7207


Abstrakt

Mammalian transient receptor potential (TRP) channels are involved in many physiologically important processes. Here, we have studied the significance of the TRPC2 channel in the regulation of rat thyroid FRTL-5 cell proliferation, migration, adhesion and invasion, using stable TRPC2 (shTRPC2) knock-down cells. In the shTRPC2 cells, proliferation was decreased due to a prolonged G1/S cell cycle phase. The tumor suppressor p53 and the cyclin-dependant kinase inhibitors p27 and p21 were upregulated. Cell invasion, adhesion and migration were also attenuated in shTRPC2 cells, probably due to decreased activity of both Rac and calpain, and a decreased secretion and activity of matrix metalloproteinase 2. The. attenuated proliferation, migration, invasion and ATP-evoked calcium entry was mimicked by over-expressing a non-conducting, truncated TRPC2 (TRPC2-DN) in wild type cells, and was reversed by overexpression of TRPC2-GFP in shTRPC2 cells. In conclusion, TRPC2 is an important regulator of rat thyroid. cell function. (c) 2013 Elsevier Ireland Ltd. All rights reserved.


Nyckelord

MMP2, TRPC2

Senast uppdaterad 2020-27-02 vid 06:23