A time-resolved fluoroimmunometric assay for the detection of microcystins, cyanobacterial peptide hepatotoxins

A1 Originalartikel i en vetenskaplig tidskrift (referentgranskad)


Interna författare/redaktörer


Publikationens författare: Mehto, Ankelo, Hinkkanen, Mikhailov, Eriksson, Spoof, Meriluoto
Publiceringsår: 2001
Tidskrift: Toxicon
Tidskriftsakronym: Toxicon
Volym: 39
Nummer: 6
Artikelns första sida, sidnummer: 831
Artikelns sista sida, sidnummer: 836
ISSN: 0041-0101
eISSN: 1879-3150


Abstrakt

An immunoassay based on the time-resolved fluorometry (TR-FIA) was developed for microcystins, cyanobacterial peptide hepatotoxins. The assay was performed in a competitive mode and it utilised the monoclonal antibodies raised against microcystin-LR, and a europium chelate of microcystin-LR as a competitive antigen. The sensitivity of the assay was 0.1microg/l. The detection method of TR-FIA was compared to a commercially available kit based on the enzyme-linked immunosorbent assay (ELISA). The same level of sensitivity could be obtained with TR-FIA (in a non-optimised system). The simplified method of TR-FIA leads to a shorter analysis time.

Senast uppdaterad 2020-30-03 vid 05:47

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