Phosphopeptide enrichment with stable spatial coordination on a titanium dioxide coated glass slide

A1 Originalartikel i en vetenskaplig tidskrift (referentgranskad)


Interna författare/redaktörer


Publikationens författare: Imanishi, Kouvonen, Smått, Heikkilä, Peuhu, Mikhailov, Ritala, Lindén, Corthals, Eriksson
Publiceringsår: 2009
Tidskrift: Rapid Communications in Mass Spectrometry
Tidskriftsakronym: Rapid Commun Mass Spectrom
Volym: 23
Nummer: 23
Artikelns första sida, sidnummer: 3661
Artikelns sista sida, sidnummer: 3667
ISSN: 0951-4198
eISSN: 1097-0231


Abstrakt

Recent advances in phosphoproteomics have established powerful tools to analyze phosphorylation events. However, their spatial localization is lost due to sample homogenization procedures prior to the analysis. Imaging mass spectrometry (IMS) has emerged as a method to visualize the spatial distribution of molecules in tissue samples, but its application is still limited to relatively abundant molecules. Due to low phosphorylation stoichiometry, direct detection and imaging of protein phosphorylation by MS has not been achieved yet. Therefore we have developed a novel phosphopeptide enrichment strategy as a potential tool for in situ affinity imaging MS (AIMS). A specific type of titanium dioxide (TiO2)-coated glass slides was designed and validated with casein tryptic digests for their ability to selectively retain phosphopeptides while maintaining their spatial coordination.

Senast uppdaterad 2020-19-02 vid 03:57