Vimentin is hyperphosphorylated in primary human fibroblasts treated with okadaic acid

A1 Originalartikel i en vetenskaplig tidskrift (referentgranskad)


Interna författare/redaktörer


Publikationens författare: Yatsunami, Fujiki, Suganuma, Yoshizawa, Eriksson, Olson, Goldman
Publiceringsår: 1991
Tidskrift: Biochemical and Biophysical Research Communications
Tidskriftsakronym: Biochem Biophys Res Commun
Volym: 177
Nummer: 3
Artikelns första sida, sidnummer: 1165
Artikelns sista sida, sidnummer: 1170
ISSN: 0006-291X


Abstrakt

Okadaic acid and dinophysistoxin-1 (35-methylokadaic acid) induced hyperphosphorylation of a 58 kDa protein in primary human fibroblasts, due to inhibition of protein phosphatase 1 and 2A activities. The protein was present in the nuclear and cytosolic fractions. Its pI was 5.3. The hyperphosphorylated protein reacted with monoclonal and polyclonal anti-vimentin antibodies, but not with anti-nucleolin antibody. Phosphorylation of vimentin was stimulated in vitro by dinophysistoxin-1 dose-dependently in the presence of protein phosphatase 2A and protein kinases.

Senast uppdaterad 2019-15-10 vid 02:51