Multifunctional Cellulose Beads and Their Interaction with Gram Positive Bacteria

A1 Journal article (refereed)


Internal Authors/Editors


Publication Details

List of Authors: Leandro S. Blachechen, Pedro Fardim, Denise F. S. Petri
Publisher: AMER CHEMICAL SOC
Publication year: 2014
Journal: Biomacromolecules
Journal acronym: BIOMACROMOLECULES
Volume number: 15
Issue number: 9
Start page: 3440
End page: 3448
Number of pages: 9
ISSN: 1525-7797
eISSN: 1526-4602


Abstract

Cellulose beads with 3 mm of diameter and high circularity were obtained by dripping cellulose solutions (5, 6, and 7 wt 96) dissolved in NaOH7%/urea12%, into HCl 2 M coagulation bath. Carboxylic groups were generated on beads surface through NaClO/NaClO2/TEMPO oxidation method, achieving total charge density of similar to 0.77 mmol/g. Pristine (CB) and oxidized (OCB) beads were characterized by means of optical images analyses, scanning electron microscopy (SEM) and compression tests. Both types of beads, CB and OCB, were used as adsorbent for poly(4-vinyl-N-pentylpyridinium) bromide, QPVP-C5, a bactericidal agent. The adsorption of QPVP-C5 on CB and OCB was evaluated by means of FTIR-ATR, UV-vis, CHN elemental analyses, and X-ray photoelectron spectroscopy (XPS). The adsorbed amount of QPVP-C5 was remarkably higher on OCB than on CB due to ionic interactions. Desorption was less than 5%. The interaction between neat OCB or OCB coated and two different amounts of QPVP-C5 and Gram-positive bacteria Micrococcus luteus was assessed by changes in turbidimetry, SEM, and elemental analyses. Bacteria adsorbed on the surface of neat OCB and weakly QPVP-C5 coated OCB due to hydrogen bonding or ion-dipole interaction. Notorious bactericidal action was observed for OCB samples coated with large amount of QPVP-C5.

Last updated on 2019-12-11 at 02:44