Optimizing the performance of tin dioxide microspheres for phosphopeptide enrichment

A1 Originalartikel i en vetenskaplig tidskrift (referentgranskad)

Interna författare/redaktörer

Publikationens författare: Alexander Leitner, Martin Sturm, Jan-Henrik Smått, Mikael Järn, Mika Lindén, Karl Mechtler, Wolfgang Lindner
Publiceringsår: 2009
Tidskrift: Analytica Chimica Acta
Tidskriftsakronym: Anal Chim Acta
Volym: 638
Nummer: 1
Artikelns första sida, sidnummer: 51
Artikelns sista sida, sidnummer: 7
ISSN: 1873-4324


Phosphopeptide enrichment based on metal oxide affinity chromatography is one of the most powerful tools for studying protein phosphorylation on a large scale. To complement existing metal oxide sorbents, we have recently introduced tin dioxide as a promising alternative. The preparation of SnO(2) microspheres by the nanocasting technique, using silica of different morphology as a template, offers a strategy to prepare materials that vary in their particle size and their porosity. Here, we demonstrate how such stannia materials can be successfully generated and their properties fine-tuned in order to obtain an optimized phosphopeptide enrichment material. We combined data from liquid chromatography-mass spectrometry experiments and physicochemical characterization, including nitrogen physisorption and energy-dispersive X-ray spectroscopy (EDX), to explain the influence of the various experimental parameters.

Senast uppdaterad 2020-20-02 vid 05:04