Dual targeted poplar ferredoxin NADP(+) oxidoreductase interacts with hemoglobin 1

A1 Originalartikel i en vetenskaplig tidskrift (referentgranskad)


Interna författare/redaktörer


Publikationens författare: Jokipii-Lukkari S, Kastaniotis AJ, Parkash V, Sundström R, Leiva-Eriksson N, Nymalm Y, Blokhina O, Kukkola E, Fagerstedt KV, Salminen TA, Läärä E, Bulow L, Ohlmeier S, Hiltunen JK, Kallio PT, Häggman H
Förläggare: ELSEVIER IRELAND LTD
Publiceringsår: 2016
Tidskrift: Plant Science
Tidskriftsakronym: PLANT SCI
Volym: 247
Artikelns första sida, sidnummer: 138
Artikelns sista sida, sidnummer: 149
Antal sidor: 12
ISSN: 0168-9452
eISSN: 1873-2259


Abstrakt

Previous reports have connected non-symbiotic and truncated hemoglobins (Hbs) to metabolism of nitric oxide (NO), an important signalling molecule involved in wood formation. We have studied the capability of poplar (Populus tremula x tremuloides) Hbs PttHb1 and PttTrHb proteins alone or with a flavin-protein reductase to relieve NO cytotoxicity in living cells. Complementation tests in a Hb-deficient, NO-sensitive yeast (Saccharomyces cerevisiae) Delta yhb1 mutant showed that neither PttHb1 nor PttTrHb alone protected cells against NO. To study the ability of Hbs to interact with a reductase, ferredoxin NADP(+) oxidoreductase PtthFNR was characterized by sequencing and proteomics. To date, by far the greatest number of the known dual-targeted plant proteins are directed to chloroplasts and mitochondria. We discovered a novel variant of hFNR that lacks the plastid presequence and resides in cytosol. The coexpression of PttHb1 and PtthFNR partially restored NO resistance of the yeast Delta yhb1 mutant, whereas PttTrHb coexpressed with PtthFNR failed to rescue growth. YFP fusion proteins confirmed the interaction between PttHb1 and PtthFNR in plant cells. The structural modelling results indicate that PttHb1 and PtthFNR are able to interact as NO dioxygenase. This is the first report on dual targeting of central plant enzyme FNR to plastids and cytosol.


Nyckelord

Dioxygenation, Dual targeting, Ferredoxin NADP(+) oxidoreductase, Hemoglobin, Nitric oxide, Poplar

Senast uppdaterad 2019-15-10 vid 03:03