Effects of heat shock and hypoxia on protein synthesis in rainbow trout (Oncorhynchus mykiss) cells

A1 Journal article (refereed)


Internal Authors/Editors


Publication Details

List of Authors: Airaksinen S, Råbergh CM, Sistonen L, Nikinmaa M
Publisher: COMPANY OF BIOLOGISTS LTD
Publication year: 1998
Journal: Journal of Experimental Biology
Journal acronym: J EXP BIOL
Volume number: 201
Issue number: 17
Start page: 2543
End page: 2551
Number of pages: 9
ISSN: 0022-0949


Abstract

We examined the effects of heat stress (from 18 degrees C to 26 degrees C) and low oxygen tension (1% O-2=1 kPa) on protein synthesis in primary cultures of hepatocytes, gill epithelial cells and fibroblast-like RTG-2 cells of rainbow trout Oncorhynchus mykiss. All these cell types displayed elevated levels of 67, 69 and 92 kDa proteins, whereas a 104 kDa protein was induced only in RTG-2 cells. Hypoxia induced a cell-type-specific response, increasing the synthesis of 36, 39 and 51 kDa proteins in the gill epithelial cells. The regulation of the heat-shock response in fish hepatocytes showed that an HSF1-like factor is involved in the transcriptional induction of the hsp70 gene. Consequently, there was a pronounced accumulation of hsp70 mRNA, Furthermore, the kinetics of activation of DNA binding and the increase in hsp70 gene expression showed a remarkable correlation, indicating that hsp70 expression is regulated at the transcriptional level in these trout cells.


Keywords

cellular stress response, gene expression, heat-shock transcription factor, Hsp70, Oncorhynchus mykiss, pH, protein synthesis, rainbow trout

Last updated on 2019-19-10 at 03:21