Functional consequences of knocking down porcine prostaglandin synthases in SK-6 swine kidney cell line

A1 Journal article (refereed)


Internal Authors/Editors


Publication Details

List of Authors: Waclawik A, Rivero-Muller A, Rahman NA, Ziecik AJ
Publisher: INST ANIMAL REPRODUCTION FOOD RESEARCH
Publication year: 2015
Journal: Reproductive Biology -Olsztyn-
Journal acronym: REPROD BIOL
Volume number: 15
Issue number: 1
Start page: 42
End page: 47
Number of pages: 6
ISSN: 1642-431X


Abstract

We hypothesized that in vitro knock-down of the previously cloned genes of prostaglandin synthases will result in a reduction of synthesis, and thus secretion of their respective products, i.e., prostaglandin (PG) E-2 or PGF(2 alpha). For this purpose, we designed short hairpin RNA (shRNA)-encoding constructs to knock down porcine mPGES-1 and PGFS (also named as AKR1CL1 in GenBank) and used them to transfect swine kidney SK-6 cells. Knocking down PGFS or mPGES-1 transcripts resulted in at least 50% inhibition of protein expression of each respective enzyme, as well as a reduction in the production of their respective prostaglandin, PGF(2 alpha), or PGE(2). These results confirmed the identities of PGFS and mPGES-1. Moreover, they illustrate a unique opportunity to use the gene knock-down constructs in primary endometrial cells in order to study their biological roles in the porcine endometrium, particularly during the establishment of pregnancy.


Keywords

Gene knock-down, Pig, Prostaglandins, ShRNA, SK-6 cells

Last updated on 2019-21-07 at 07:17