Evaluation of Antibacterial and Anti-biofilm Activities of Cinchona Alkaloid Derivatives against Staphylococcus aureus

A1 Journal article (refereed)


Internal Authors/Editors


Publication Details

List of Authors: Skogman ME, Kujala J, Busygin I, Leino R, Vuorela PM, Fallarero A
Publisher: NATURAL PRODUCTS INC
Publication year: 2012
Journal: Natural Product Communications
Journal acronym: NAT PROD COMMUN
Volume number: 7
Issue number: 9
Start page: 1173
End page: 1176
Number of pages: 4
ISSN: 1934-578X


Abstract

Bacterial biofilms are resistant to most of the commonly available antibacterial chemotherapies. Thus, an enormous need exists to meet the demands of effective anti-biofilm therapy. In this study, a small library of cinchona alkaloids, including the naturally occurring compounds cinchonidine and cinchonine, as well as various synthetic derivatives and analogues was screened for antibacterial and anti-biofilm activity against the Staphylococcus aureus biofilm producing strain ATCC 25923. Two methods were used to evaluate activity against biofilms, namely crystal violet staining to measure biomass and resazurin assay to measure biofilms viability. Cinchonidine was found to be inactive, whereas a synthetic derivative, 11-triphenylsilyl-10,11-dihydrocinchonidine (11-TPSCD), was effective against planktonic bacteria as well as in preventing biofilm formation at low micromolar concentrations. Higher concentrations were required to eradicate mature biofilms.


Keywords

Antimicrobial, Biofilms, ChemGPS-NP, Cinchona alkaloid, Fluorescence microscopy, Staphylococcus aureus

Last updated on 2019-10-12 at 02:42