Spheroid culture of LuCaP 136 patient-derived xenograft enables versatile preclinical models of prostate cancer

A1 Originalartikel i en vetenskaplig tidskrift (referentgranskad)


Interna författare/redaktörer


Publikationens författare: Valta MP, Zhao HJ, Saar M, Tuomela J, Nolley R, Linxweiler J, Sandholm J, Lehtimaki J, Harkonen P, Coleman I, Nelson PS, Corey E, Peehl DM
Förläggare: SPRINGER
Publiceringsår: 2016
Tidskrift: Clinical and Experimental Metastasis
Tidskriftsakronym: CLIN EXP METASTAS
Volym: 33
Nummer: 4
Artikelns första sida, sidnummer: 325
Artikelns sista sida, sidnummer: 337
Antal sidor: 13
ISSN: 0262-0898
eISSN: 1573-7276


Abstrakt

LuCaP serially transplantable patient-derived xenografts (PDXs) are valuable preclinical models of locally advanced or metastatic prostate cancer. Using spheroid culture methodology, we recently established cell lines from several LuCaP PDXs. Here, we characterized in depth the features of xenografts derived from LuCaP 136 spheroid cultures and found faithful retention of the phenotype of the original PDX. In vitro culture enabled luciferase transfection into LuCaP 136 spheroids, facilitating in vivo imaging. We showed that LuCaP 136 spheroids formed intratibial, orthotopic, and subcutaneous tumors when re-introduced into mice. Intratibial tumors responded to castration and were highly osteosclerotic. LuCaP 136 is a realistic in vitro-in vivo preclinical model of a subtype of bone metastatic prostate cancer.


Nyckelord

Androgen-dependence, Bone metastasis, Osteosclerosis

Senast uppdaterad 2019-24-09 vid 04:55

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