Studies Related to Norway Spruce Galactoglucomannans: Chemical Synthesis, Conformation Analysis, NMR Spectroscopic Characterization, and Molecular Recognition of Model Compounds

A1 Journal article (refereed)


Internal Authors/Editors


Publication Details

List of Authors: Filip S. Ekholm, Ana Ardá, Patrik Eklund, Sabine André, Hans-Joachim Gabius, Jesús Jiménez-Barbero, and Reko Leino
Publisher: WILEY-V C H VERLAG GMBH
Publication year: 2012
Journal: Chemistry - A European Journal
Journal acronym: CHEM-EUR J
Volume number: 18
Issue number: 45
Start page: 14392
End page: 14405
Number of pages: 14
ISSN: 0947-6539


Abstract

Galactoglucomannan (GGM) is a polysaccharide mainly consisting of mannose, glucose, and galactose. GGM is the most abundant hemicellulose in the Norway spruce (Picea abies), but is also found in the cell wall of flax seeds, tobacco plants, and kiwifruit. Although several applications for GGM polysaccharides have been developed in pulp and paper manufacturing and the food and medical industries, attempts to synthesize and study distinct fragments of this polysaccharide have not been reported previously. Herein, the synthesis of one of the core trisaccharide units of GGM together with a less-abundant tetrasaccharide fragment is described. In addition, detailed NMR spectroscopic characterization of the model compounds, comparison of the spectral data with natural GGM, investigation of the acetyl-group migration phenomena that takes place in the polysaccharide by using small model compounds, and a binding study between the tetrasaccharide model fragment and a galactose-binding protein (the toxin viscumin) are reported.


Keywords

conformation analysis, galactoglucomannan, molecular recognition, NMR spectroscopy, oligosaccharide synthesis

Last updated on 2019-13-11 at 02:47