Selective integrin endocytosis is driven by interactions between the integrin α-chain and AP2

A1 Journal article (refereed)


Internal Authors/Editors


Publication Details

List of Authors: De Franceschi N, Arjonen A, Elkhatib N, Denessiouk K, Wrobel AG, Wilson TA, Pouwels J, Montagnac G, Owen DJ, Ivaska J
Publisher: NATURE PUBLISHING GROUP
Publication year: 2016
Journal: Nature Structural and Molecular Biology
Journal acronym: NAT STRUCT MOL BIOL
Volume number: 23
Issue number: 2
Start page: 172
End page: 179
Number of pages: 10
ISSN: 1545-9993
eISSN: 1545-9985


Abstract

Integrins are heterodimeric cell-surface adhesion molecules comprising one of 18 possible a-chains and one of eight possible beta-chains. They control a range of cell functions in a matrix-and ligand-specific manner. Integrins can be internalized by clathrin-mediated endocytosis (CME) through b subunit-based motifs found in all integrin heterodimers. However, whether specific integrin heterodimers can be selectively endocytosed was unknown. Here, we found that a subset of alpha subunits contain an evolutionarily conserved and functional Yxx phi. motif directing integrins to selective internalization by the most abundant endocytic clathrin adaptor, AP2. We determined the structure of the human integrin alpha(4)-tail motif in complex with the AP2 C-mu 2 subunit and confirmed the interaction by isothermal titration calorimetry. Mutagenesis of the motif impaired selective heterodimer endocytosis and attenuated integrin-mediated cell migration. We propose that integrins evolved to enable selective integrin-receptor turnover in response to changing matrix conditions.

Last updated on 2019-18-08 at 06:03