Characterisation of genes transcriptionally upregulated in the liver of sand goby (Pomatoschistus minutus) by 17 alpha-ethinyloestradiol: Identification of distinct vitellogenin and zona radiata protein transcripts

A1 Originalartikel i en vetenskaplig tidskrift (referentgranskad)


Interna författare/redaktörer


Publikationens författare: Humble JL, Hands E, Saaristo M, Lindström K, Lehtonen KK, de Cerio OD, Cancio I, Wilson G, Craft JA
Förläggare: PERGAMON-ELSEVIER SCIENCE LTD
Publiceringsår: 2013
Tidskrift: Chemosphere
Tidskriftsakronym: CHEMOSPHERE
Volym: 90
Nummer: 11
Artikelns första sida, sidnummer: 2722
Artikelns sista sida, sidnummer: 2729
Antal sidor: 8
ISSN: 0045-6535


Abstrakt

The sand goby (Pomatoschistus minutus), is a marine and estuarine teleost that is used in environmental, reproductive and behavioural studies of oestrogenic endocrine disruption. The xeno-oestrogen, 17 alpha-ethinyloestradiol (EE2), induces expression of egg proteins vitellogenin (VTG) and zona radiata protein (ZRP) in male fish and impairs reproduction. Multiple forms of VTG and ZRP genes are found in other teleost species, yet the characteristics of VTG and ZRP in the sand goby are unknown. In this investigation, Suppressive Subtractive Hybridization was used to isolate cDNA fragments from liver, identified as belonging to 11 distinct sand goby genes, suggesting that these genes are transcriptionally upregulated by EE2. Assembly of these fragments revealed three VTG genes which shared homology with VTG classes A, B and C in other fish and two ZRP genes sharing homology with ZRP classes Ba and Bb. RTqPCR of RNA from the sand goby liver was used to show that these VTGs and ZRPs were present in low levels in control males and high levels in mature females. Exposure of males to a concentration of 11 ng L-1 EE2 caused a significant increase in all VTG and ZRP transcript levels. The identification of these egg protein transcripts and the development of validated assays for their quantification will facilitate future work with this useful model species.


Nyckelord

17 alpha-ethinyloestradiol, Endocrine disruption, Sand goby, Vitellogenin, Zona radiata protein

Senast uppdaterad 2020-22-01 vid 04:48