Investigation of protein binding with all solid-state ion-selective electrodes

A1 Originalartikel i en vetenskaplig tidskrift (referentgranskad)


Interna författare/redaktörer


Publikationens författare: Alok Prabhu, Johan Bobacka, Ari Ivaska, Kalle Levon
Förläggare: WILEY-V C H VERLAG GMBH
Publiceringsår: 2013
Tidskrift: Electroanalysis
Tidskriftsakronym: ELECTROANAL
Volym: 25
Nummer: 8
Artikelns första sida, sidnummer: 1887
Artikelns sista sida, sidnummer: 1894
Antal sidor: 8
ISSN: 1040-0397
eISSN: 1521-4109


Abstrakt

A potentiometric sensor for studying charge based adsorption of proteins was created using a single-piece polyaniline-PVC ion-selective electrode (ISE). Three different ISEs, two for Na+ and one for Cl- ion determination, were studied. The Na+-ISEs consisted of a neutral calixarene-based ionophore and one with a charged carrier dinonylnapthalenesulfonic acid (DNNSA) whereas for the Cl- ISE, an anion exchanger tridodecylmethylammonium chloride (TDDMA(+)Cl(-)), was used. The Na+ ISE with DNNSA as the charged carrier was successfully able to discriminate the binding of two different proteins (bovine serum albumin and lysozyme) based on their intrinsic charge.


Nyckelord

Conducting polymers, Electrochemical biosensors, Ion-selective electrodes, Potentiometry, Protein adsorption

Senast uppdaterad 2019-06-12 vid 04:39