CELL-TRANSFORMATION BY C-HA-RAS(VAL12) ONCOGENE IS ACCOMPANIED BY A DECREASE IN HISTONE H1-DEGREES AND AN INCREASE IN NUCLEOSOMAL REPEAT LENGTH

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Publikationens författare: LAITINEN J, SISTONEN L, ALITALO K, HÖLTTÄ E
Förläggare: WILEY-LISS
Publiceringsår: 1995
Tidskrift: Journal of Cellular Biochemistry
Tidskriftsakronym: J CELL BIOCHEM
Volym: 57
Nummer: 1
Artikelns första sida, sidnummer: 1
Artikelns sista sida, sidnummer: 11
Antal sidor: 11
ISSN: 0730-2312


Abstrakt

The activated c-Ha-ras(Val12) oncogene is often involved in the genesis of human malignancies. We show here that in c-Ha-ras(Val12) oncogene-transformed mouse NIH 3T3 fibroblasts the copy number and expression level of the mutant ras oncogene correlates with the degree of chromatin decondensation, as assessed by micrococcal nuclease (MNase) and DNase I digestion. MNase and DNase I analyses further revealed that the nucleosomal repeat lengths were different in the normal and ras oncogene-transformed cells, 162.3 bp and 178.1 bp, respectively. These chromatin changes were accompanied by alterations in the content of histone H1 degrees. Furthermore, using DNase I as a probe, we discovered that serum stimulation of normal and transformed cells, synchronized by serum starvation, induces rapid reversible changes in the structure of bulk chromatin that may be linked to transcriptional activation. Our data thus indicate that cell transformation by ras is associated with specific changes in chromatin structure that make it more vulnerable, and prone to additional mutations characteristic of cancer development in vivo.


Nyckelord

CELL TRANSFORMATION, C-HA-RAS(VAL12) ONCOGENE, CHROMATIN STRUCTURE, HISTONE H1-DEGREES, NUCLEOSOMAL REPEAT LENGTH, SERUM STIMULATION

Senast uppdaterad 2019-16-06 vid 04:31

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