Evaluation of acid methanolysis for analysis of wood hemicelluloses and pectins

A1 Originalartikel i en vetenskaplig tidskrift (referentgranskad)

Interna författare/redaktörer

Publikationens författare: Bertaud F, Sundberg A, Holmbom B
Publiceringsår: 2002
Tidskrift: Carbohydrate Polymers
Tidskriftsakronym: CARBOHYD POLYM
Volym: 48
Nummer: 3
Artikelns första sida, sidnummer: 319
Artikelns sista sida, sidnummer: 324
Antal sidor: 6
ISSN: 0144-8617


The efficiency of acid methanolysis toward cleavage of glucuronosyl linkages in hemicelluloses and pectins was studied. Two aldo-uronic acids (a dimer and a tetramer) were submitted to methanolysis with different acidic strength and methanolysis time. The aldo-uronic acid tetramer was only affected by the acidity of the medium, whereas the dimer was sensitive towards both reaction time and acidity. It was concluded that methanolysis of 0.5-1 mg of model compounds with 2 ml of 2 M HCl/MeOH at 100degreesC for 3-5 h was the best compromise between good cleavage of the glucuronosyl bonds ( approximate to 70%, mol/mol) and little degradation of the uronic acid released (below 20%, mol/ mol). The acid methanolysis of reference oligo- and polysaccharides verified the good accuracy of the method. The application of acid methanolysis on mechanical pulp fibres was tested on a thermomechanical pulp. (C) 2002 Elsevier Science Ltd. All rights reserved.


fibres, glucuronosyl linkage, hemicelluloses, methanolysis, pectins, polysaccharide, Pulp, uronic acid, wood

Senast uppdaterad 2019-10-12 vid 02:54