Evaluation of acid methanolysis for analysis of wood hemicelluloses and pectins

A1 Journal article (refereed)


Internal Authors/Editors


Publication Details

List of Authors: Bertaud F, Sundberg A, Holmbom B
Publisher: ELSEVIER SCI LTD
Publication year: 2002
Journal: Carbohydrate Polymers
Journal acronym: CARBOHYD POLYM
Volume number: 48
Issue number: 3
Start page: 319
End page: 324
Number of pages: 6
ISSN: 0144-8617


Abstract

The efficiency of acid methanolysis toward cleavage of glucuronosyl linkages in hemicelluloses and pectins was studied. Two aldo-uronic acids (a dimer and a tetramer) were submitted to methanolysis with different acidic strength and methanolysis time. The aldo-uronic acid tetramer was only affected by the acidity of the medium, whereas the dimer was sensitive towards both reaction time and acidity. It was concluded that methanolysis of 0.5-1 mg of model compounds with 2 ml of 2 M HCl/MeOH at 100degreesC for 3-5 h was the best compromise between good cleavage of the glucuronosyl bonds ( approximate to 70%, mol/mol) and little degradation of the uronic acid released (below 20%, mol/ mol). The acid methanolysis of reference oligo- and polysaccharides verified the good accuracy of the method. The application of acid methanolysis on mechanical pulp fibres was tested on a thermomechanical pulp. (C) 2002 Elsevier Science Ltd. All rights reserved.


Keywords

fibres, glucuronosyl linkage, hemicelluloses, methanolysis, pectins, polysaccharide, Pulp, uronic acid, wood

Last updated on 2019-09-12 at 03:35