Liquid chromatographic-tandem mass spectrometric method for the plant lignan 7-hydroxymatairesinol and its potential metabolites in human plasma

A1 Originalartikel i en vetenskaplig tidskrift (referentgranskad)

Interna författare/redaktörer

Publikationens författare: Smeds A, Hakala K
Publiceringsår: 2003
Tidskrift: Journal of Chromatography B
Tidskriftsakronym: J CHROMATOGR B
Volym: 793
Nummer: 2
Artikelns första sida, sidnummer: 297
Artikelns sista sida, sidnummer: 308
Antal sidor: 12
ISSN: 1570-0232


A HPLC-MS-MS method was developed for the determination of the plant lignan 7-hydroxymatairesinol and its potential metabolites matairesinol, oxomatairesinol, alpha-conidendrin, 7-hydroxyenterolactone, enterodiol, and enterolactone in human plasma. The method included sample cleanup by solid-phase extraction (SPE) and analysis using a PE Sciex API3000 triple quadrupole mass spectrometer with electrospray ionisation. The lignans were quantified using two deuterated internal standards. They showed good chromatographic linearity, analysis repeatability, and SPE recovery in the presence of plasma. In pooled plasma and in plasma samples collected from two individual subjects lignan glucuronides and sulfates were enzymatically hydrolysed to free lignans and then analysed. All the lignans could be detected in the samples. (C) 2003 Elsevier B.V. All rights reserved.


7-hydroxyenterolactone, enterodiol, enterolactone, mammalian lignans

Senast uppdaterad 2020-28-01 vid 03:07