Advanced cellulose fibers for efficient immobilization of enzymes

A1 Originalartikel i en vetenskaplig tidskrift (referentgranskad)


Interna författare/redaktörer


Publikationens författare: Iris Beatriz Vega Erramuspe, Elnaz Fazeli, Tuomas Näreoja, Jani Trygg, Pekka Hänninen, Thomas Heinze, Pedro Fardim
Förläggare: ACS
Publiceringsår: 2016
Tidskrift: Biomacromolecules
Tidskriftsakronym: Biomacromolecules
Volym: 17
Nummer: 10
Artikelns första sida, sidnummer: 3188
Artikelns sista sida, sidnummer: 3197
eISSN: 1526-4602


Abstrakt

Biocatalytic pulp fibers were prepared using surface functionalization of bleached kraft pulp with amino groups (F) and further immobilization of a cross-linked glucose oxidase (G*) from Aspergillus niger. The cross-linked enzymes (G*) were characterized using X-ray spectroscopy, Fourier transform infrared spectroscopy, dynamic scanning calorimetry, and dynamic light scattering. According to standard assays, the G* content on the resulting fibers (FG*) was of 11 mg/g of fiber, and enzyme activity was of 215 U/g. The results from confocal- and stimulated emission
depletion microscopy techniques demonstrated that glucose oxidase do not penetrate the interlayers of fibers. The benefit of pulp fiber functionalization was evident in the present case, as the introduction of amino groups allowed the immobilization of larger amount of enzymes and rendered more efficient systems. Using the approach described on this paper, several advanced materials from wood pulp fibers and new bioprocesses might be developed by selecting the correct enzyme for the target applications.


Nyckelord

biocatalysis, Bioeconomy, Cellulose fibres, cellulose functional fibres, enzyme immobilization

Senast uppdaterad 2019-20-08 vid 05:07